مورد إلكتروني
Evaluating full-thickness skin grafts in intraperitoneal onlay mesh position versus onlay position in mice
| العنوان: | Evaluating full-thickness skin grafts in intraperitoneal onlay mesh position versus onlay position in mice |
|---|---|
| بيانات النشر: | Umeå universitet, Kirurgi Umeå universitet, Kirurgi Umeå universitet, Kirurgi 2018 |
| تفاصيل مُضافة: | Winsnes, Annika Gunnarsson, Ulf Falk, Peter Stark, Birgit Moskaug, Jan Ø. Strigård, Karin |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | BACKGROUND: Importance: Hernia surgery requires reinforcement material with few side effects when used in the intraperitoneal position. Autologous skin grafting may meet this requirement, but animal experiments are obligatory before being applied in humans. OBJECTIVE: To compare survival and effects of isogeneic full-thickness skin grafts in the intraperitoneal onlay mesh (IPOM) position in mice, with a control group using the onlay position. Primary end point was graft survival and secondary end point adhesion formation and inflammation through NF-κB activity. METHODS: Design: Intervention study with 8-week follow-up in accordance with ARRIVE criteria, performed between 2015 and 2016. SETTING: Animal laboratory. PARTICIPANTS: Transgenic C57BL/6 mice with isogeneic background were used. Recipients were female wild-type phenotype mice >3 mo (n = 24). Donors were male or female mice >7 mo, with phenotype-positive for the luciferase gene (n = 20) or positive for NF-κB-luciferase gene (n = 4). INTERVENTION: Full-thickness skin was grafted in the IPOM position and compared with grafts in the onlay position as controls. Survival was evaluated by regular longitudinal postoperative luminescence imaging over 8 wk. Adherence formation was evaluated macroscopically after sacrifice. Inflammation of full-thickness skin grafts in IPOM position of NF-κB mice was evaluated in four additional mice. Main outcome and measure: Survival of grafts, evaluated by luminescence. RESULTS: Ten animals received grafts in the IPOM position, and 10 in the onlay position as controls. Graft survival after 8 wk was 100% (20/20). Average luminescence at the end of the 8-week period was 999,597 flux (min 162,800, max 2,521,530) in the IPOM group (n = 10) and 769,708 flux (min 76,590, max 2,164,080) in the onlay control group (n = 10). No adhesions requiring sharp dissection (Jenkins' scale >2) were seen. Four animals with grafts in the IPOM position showed peak inflammation (NF-κB activity) 5 |
| مصطلحات الفهرس: | Abdominal wall reinforcement, Acellular scaffold, Autologous full-thickness skin graft, Hernia repair, IPOM/onlay, Intraperitoneal, Isogeneic, Surgery, Kirurgi, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1016.j.jss.2018.04.041 |
| URL: | Journal of Surgical Research, 0022-4804, 2018, 230, s. 155-163 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | UPE oai:DiVA.org:umu-151807 doi:10.1016/j.jss.2018.04.041 PMID 30100033 ISI:000441170900023 Scopus 2-s2.0-85047625025 1234716687 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1234716687 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1016.j.jss.2018.04.041 |
|---|