مورد إلكتروني
Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity
| العنوان: | Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity |
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| بيانات النشر: | KTH, Glykovetenskap KTH, Beräkningsbiologi, CB 2009 |
| تفاصيل مُضافة: | Fugelstad, Johanna Bouzenzana, Jamel Djerbi, Soraya Guerriero, Gea Ezcurra, Inés Teeri, Tuula T. Arvestad, Lars Bulone, Vincent |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Cellulose biosynthesis is a vital but yet poorly understood biochemical process in Oomycetes. Here, we report the identification and characterization of the cellulose synthase genes (CesA) from Saprolegnia monoica. Southern blot experiments revealed the occurrence of three CesA homologues in this species and phylogenetic analyses confirmed that Oomycete CesAs form a clade of their own. All gene products contained the D,D,D,QXXRW signature of most processive glycosyltransferases, including cellulose synthases. However, their N-terminal ends exhibited Oomycete-specific domains, i.e. Pleckstrin Homology domains, or conserved domains of an unknown function together with additional putative transmembrane domains. Mycelial growth was inhibited in the presence of the cellulose biosynthesis inhibitors 2,6-dichlorobenzonitrile or Congo Red. This inhibition was accompanied by a higher expression of all CesA genes in the mycelium and increased in vitro glucan synthase activities. Altogether, our data strongly suggest a direct involvement of the identified CesA genes in cellulose biosynthesis. QC 20100525 |
| مصطلحات الفهرس: | Cellulose synthase genes, Cell wall biosynthesis, Congo Red, 2, 6-Dichlorobenzonitrile (DCB), Oomycetes, Saprolegnia monoica, fungus saprolegnia, acetobacter-xylinum, catalytic subunit, chitin, synthase, plasma-membrane, wall chemistry, cell-walls, congo red, biosynthesis, dna, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1016.j.fgb.2009.07.001 |
| URL: | Fungal Genetics and Biology, 1087-1845, 2009, 46:10, s. 759-767 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:kth-18715 0000-0001-9080-3031 0000-0001-5341-1733 0000-0003-2809-4160 doi:10.1016/j.fgb.2009.07.001 PMID 19589393 ISI:000269368000005 Scopus 2-s2.0-68549136474 1235003115 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1235003115 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1016.j.fgb.2009.07.001 |
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