مورد إلكتروني
Anticancer properties of the fatty acid synthase inhibitor TVB-3166 on oral squamous cell carcinoma cell lines
| العنوان: | Anticancer properties of the fatty acid synthase inhibitor TVB-3166 on oral squamous cell carcinoma cell lines |
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| بيانات النشر: | Elsevier 2020 |
| تفاصيل مُضافة: | Aquino, I. G. (Iara Gonçalves de) Bastos, D. C. (Débora Campanella) Cuadra-Zelaya, F. J. (Florence Juana Maria) Teixeira, I. F. (Isadora Ferrari) Salo, T. (Tuula) Coletta, R. D. (Ricardo Della) Graner, E. (Edgard) |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Objective: Fatty acid synthase (FASN) is overexpressed in several human cancers, including oral squamous cell carcinoma (OSCC). TVB-3166 is a recently described FASN inhibitor with antitumor effects and potential clinical relevance. The objective of this study was to evaluate the effects of TVB-3166 on OSCC cell lines. Materials and methods: The OSCC cell line SCC-9 modified to express ZsGreen (ZsG) (SCC-9 ZsG) and its in vivo selected metastatic derivative LN-1A were used to evaluate anticancer properties of TVB-3166. Cell viability was determined using MTT assays and proliferation determined by cell counting in a Neubauer chamber. Cell death and cell cycle progression were analyzed by Annexin V-PE/7-ADD-PerCP labeling and PI staining, respectively. Cell migration was assayed by scratch assays and cell adhesion using myogel. Production of FASN, p-AKT, CPT1-α, and epithelial-mesenchymal transition (EMT) markers were examined by Western blotting. Results: TVB-3166 significantly reduced cell viability and proliferation, promoted cell cycle arrest and apoptosis, and increased adhesion to myogel in both OSCC cell lines. Finally, the drug reduced SCC-9 ZsG migration. Conclusion: Our results demonstrated that TVB-3166 has anticancer effects on both SCC-9 ZsG and its metastatic version LN-1A, which are worthy of investigation in preclinical models for OSCC. |
| مصطلحات الفهرس: | Cell lines, Fatty acid synthase, Oral squamous cell carcinoma, TVB-3166, info:eu-repo/semantics/article, info:eu-repo/semantics/acceptedVersion |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess © 2020 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. https://creativecommons.org/licenses/by-nc-nd/4.0 |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | OUX oai:oulu.fi:nbnfi-fe2020050625352 urn:nbn:fi-fe2020050625352 1244881535 |
| المصدر المساهم: | UNIV OF OULU From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1244881535 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |