مورد إلكتروني
Killed whole-genome reduced-bacteria surface-expressed coronavirus fusion peptide vaccines protect against disease in a porcine model
| العنوان: | Killed whole-genome reduced-bacteria surface-expressed coronavirus fusion peptide vaccines protect against disease in a porcine model |
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| بيانات النشر: | National Academy of Sciences 2021-04-15 |
| تفاصيل مُضافة: | Maeda, Denicar Lina Nascimento Fabris Tian, Debin Yu, Hanna Dar, Nakul Rajasekaran, Vignesh Meng, Sarah Mahsoub, Hassan Sooryanarain, Harini Wang, Bo Heffron, C. Lynn Hassebroek, Anna LeRoith, Tanya Meng, Xiang-Jin Zeichner, Steven L. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | As the coronavirus disease 2019 (COVID-19) pandemic rages on, it is important to explore new evolution-resistant vaccine antigens and new vaccine platforms that can produce readily scalable, inexpensive vaccines with easier storage and transport. We report here a synthetic biology-based vaccine platform that employs an expression vector with an inducible gram-negative autotransporter to express vaccine antigens on the surface of genome-reduced bacteria to enhance interaction of vaccine antigen with the immune system. As a proof-of-principle, we utilized genome-reduced Escherichia coli to express SARS-CoV-2 and porcine epidemic diarrhea virus (PEDV) fusion peptide (FP) on the cell surface, and evaluated their use as killed whole-cell vaccines. The FP sequence is highly conserved across coronaviruses; the six FP core amino acid residues, along with the four adjacent residues upstream and the three residues downstream from the core, are identical between SARS-CoV-2 and PEDV. We tested the efficacy of PEDV FP and SARS-CoV-2 FP vaccines in a PEDV challenge pig model. We demonstrated that both vaccines induced potent anamnestic responses upon virus challenge, potentiated interferon-γ responses, reduced viral RNA loads in jejunum tissue, and provided significant protection against clinical disease. However, neither vaccines elicited sterilizing immunity. Since SARS-CoV-2 FP and PEDV FP vaccines provided similar clinical protection, the coronavirus FP could be a target for a broadly protective vaccine using any platform. Importantly, the genome-reduced bacterial surface-expressed vaccine platform, when using a vaccine-appropriate bacterial vector, has potential utility as an inexpensive, readily manufactured, and rapid vaccine platform for other pathogens. |
| مصطلحات الفهرس: | vaccine, genome-reduced bacteria vaccine platform, fusion peptide, porcine epidemic diarrhea virus (PEDV), SARS-CoV-2, Article - Refereed |
| DOI: | 10.1073.pnas.2025622118 |
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| الإتاحة: | Open access content. Open access content Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 |
| ملاحظة: | en_US |
| أرقام أخرى: | VPI oai:vtechworks.lib.vt.edu:10919/104126 doi:10.1073/pnas.2025622118 1260130602 |
| المصدر المساهم: | VIRGINIA TECH From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1260130602 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1073.pnas.2025622118 |
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