مورد إلكتروني
Comparison of two different primer sets for detection of Pasteurella caballi in bronchoalveolar lavage fluids samples from thoroughbred Arabian foals, using PCR.
| العنوان: | Comparison of two different primer sets for detection of Pasteurella caballi in bronchoalveolar lavage fluids samples from thoroughbred Arabian foals, using PCR. |
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| عناروين إضافية: | Usporedba vrijednosti dvaju različitih kompleta početnica za dokaz vrste Pasteurella caballi lančanom reakcijom polimerazom u uzorcima bronhoalveolarnog ispirka ždrebadi čistokrvnoga arapskog konja. |
| المصدر: | Veterinarski arhiv; ISSN 0372-5480 (Print); ISSN 1331-8055 (Online); Volume 86; Issue 2 |
| بيانات النشر: | Faculty of Veterinary Medicine University of Zagreb 2016 |
| تفاصيل مُضافة: | Zafer Sayin Osman Erganis Asli Sakmanoglu Hasan H. Hadimli Yasemin Pinarkara Mehmet Maden Huda J. G. Al Shattrawi |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | In the present study, Pasteurella caballi (P. caballi) was isolated and identified in bronchoalveolar lavage fluid and lung samples from thoroughbred Arabian foals using conventional microbiological methods. Subsequently, the ability of two different PCR primer sets was evaluated for detection and confirmation of P. caballi. Primer sets 1 and 2, targeting the 16S rRNA gene of P. caballi, were designed using the Primer 3 and Primer-BLAST programs, respectively. PCR was performed to confirm P. caballi strains and to detect it directly in the bronchoalveolar lavage fluid and lung samples. In total, 35 Pasteurella spp. were isolated from 25 (38.4 %) of 65 bronchoalveolar lavage fluid samples, and 10 (58.8 %) of 17 lung samples. These strains were identified as P. caballi based on conventional microbiological and biochemical characteristics. The sensitivities of primers 1 and 2 were determined to be 100 % to confirm cultured P. caballi strains. However, the specificity of P. caballi detection was lower with primer set-1 than primer set-2 in bronchoalveolar lavage fluid and lung samples. The sensitivity and specificity of primer set-2 were confirmed by gene sequence analysis. This study indicates that the 16S rRNA-PCR method, using primer set-2, provides a rapid and accurate tool for the detection and confirmation of P. caballi isolates in bronchoalveolar lavage fluid and lung samples from foals. Pasteurella caballi (P. caballi) izdvojena je i identificirana u uzorcima bronhoalveolarnog ispirka i tkiva pluća ždrebadi čistokrvnoga arapskoga konja uobičajenim mikrobiološkim postupcima. Potom je istražena vrijednost dvaju različitih kompleta početnica za dokaz i potvrdu vrste P. caballi lančanom reakcijom polimerazom. Početnica 1 za dokaz gena 16S rRNA P. caballi bila je pripremljena upotrebom programa Primer 3, a početnica 2 upotrebom programa Primer-BLAST. Lančana reakcija polimerazom rabljena je za potvrdu sojeva vrste P. caballi i za njezin izravni dokaz u uzorcima bronhoalveolarnog ispirka i plućnoga tkiva. Ukupno je bilo izdvojeno 35 izolata Pasteurella spp. iz 25 (38,4 %) od 65 pretraženih uzoraka bronhoalveolarnog ispirka i 10 (58,8 %) izolata iz 17 uzoraka plućnoga tkiva. Ti su sojevi bili identificirani na osnovi poznatih mikrobioloških i biokemijskih značajki. Početnice 1 i 2 pokazale su 100 %-tnu osjetljivost za identifikaciju uzgojenih sojeva. Međutim, početnica set-1 za dokaz vrste P. caballi bila je slabije specifičnosti od početnice set-2 pri pretrazi uzoraka bronhoalveolarnog ispirka i plućnoga tkiva. Osjetljivost i specifičnost početnice 2 bila je povrđena analizom genske sekvencije. Istraživanje pokazuje da početnica set-2 za lančanu reakciju polimerazom za dokaz 16S rRNA pruža brz i točan alat za dokazivanje i potvrdu izolata vrste P. caballi u bronhoalveolarnom ispirku i plućnom tkivu ždrebadi. |
| مصطلحات الفهرس: | Arabian foals; Pasteurella caballi; polymerase chain reaction; primer-BLAST; primer 3; 16S rRNA, arapski konj; ždrebad; Pasteurella caballi; lančana reakcija polimerazom; Primer-BLAST; Primer 3; 16S rRNA, text, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess Full-text papers can be used for personal or educational purposes only. Authors' and publisher's copyrights must be acknowledged. |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | HRCAK oai:hrcak.srce.hr:156705 1280900616 |
| المصدر المساهم: | HRCAK PORTAL ZNANSTVENIH CASOPISA REPUB From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1280900616 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |