مورد إلكتروني
Rapid and visual loop mediated isothermal amplification (LAMP) test for the detection of Brucella spp. and its applicability in epidemiology of bovine brucellosis.
| العنوان: | Rapid and visual loop mediated isothermal amplification (LAMP) test for the detection of Brucella spp. and its applicability in epidemiology of bovine brucellosis. |
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| عناروين إضافية: | Brzo i vidljivo petljom posredovano izotermno umnažanje za dokazivanje Brucella spp. i njegova primjena u epidemiologiji bruceloze goveda. |
| المصدر: | Veterinarski arhiv; ISSN 0372-5480 (Print); ISSN 1331-8055 (Online); Volume 86; Issue 1 |
| بيانات النشر: | Faculty of Veterinary Medicine University of Zagreb 2016 |
| تفاصيل مُضافة: | Kumaragurubaran Karthik Rajesh Rathore Prasad Thomas Konasagara N. Viswas Rajesh K. Agarwal Valsala Rekha Ramesh V. Jagapur Kuldeep Dhama |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Brucellosis is a devastating disease, once an abortion storm starts in a herd, and hence early diagnosis is important. Loop mediated isothermal amplification (LAMP) has enlightened the darker part of the diagnostic world by its simplicity and swiftness, and has the advantage of working in isothermal conditions so that it can be applied to the field. LAMP for Brucella spp., that already exists has not been exploited for visual detection and the time needed for completion of the reaction has not been reduced. In the present study, an attempt was made to seal these cracks by developing a fast and visually detectable LAMP for Brucella spp., which needs only 30 min detection time, and SYBR green aided easier result visualization. The LAMP test targeting the omp25 gene was found to be highly sensitive and specific for detecting Brucella spp. Comparison of the LAMP test with the available PCR assay revealed that the LAMP test was more sensitive than PCR, following testing of 438 field samples of cattle origin, and it showed prevalence of Brucella of 14.7 % in different parts of India. Being highly sensitive, specific and speedy, the standardized visual LAMP test can be widely used for epidemiological surveys of this economically important and zoonotic pathogen. Bruceloza je razorna bolest kad se u stadu pojavi u obliku pobačaja što iziskuje brzo postavljanje dijagnoze. Upotreba metode nazvane „petljom posredovano izotermno umnažanje“ (engl. loop mediated isothermal amplification, LAMP)“ omogućila je poboljšanje dijagnostike svojom jednostavnošću i brzinom, a prednost joj je da se može izvesti na postojanoj temperaturi pa se može primijeniti u terenskim uvjetima. U dosada razvijenim postupcima LAMP-a nije bio rabljen vizualni dokaz i nije se uspjelo skratiti vrijeme reakcije. U ovom istraživanju pokušalo se ukloniti te nedostatke razvitkom brzog postupka za vizualni dokaz Brucella spp., za što je potrebno samo 30 minuta i SYBR zeleno. LAMP test za dokaz gena omp25 pokazao se visoko osjetljivim i specifičnim za dokaz Brucella spp. Pretraga 438 terenskih uzoraka podrijetlom od goveda pokazala je da je LAMP osjetljiviji od raspoloživih testova PCR-a. Tim je testom dokazana prevalencija bruceloze od 14,7 % u različitim dijelovima Indije. Kao vrlo osjetljiv, specifičan i brz, LAMP test se može naširoko upotrijebiti u epidemiološkim istraživanjima bruceloze. |
| مصطلحات الفهرس: | visual loop mediated isothermal amplification test; Brucella; loop primers; omp25; SYBR green, petljom posredovano izotermno umnažanje; brucele; početnice; omp25; SYBR zeleno, text, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess Full-text papers can be used for personal or educational purposes only. Authors' and publisher's copyrights must be acknowledged. |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | HRCAK oai:hrcak.srce.hr:153491 1280900796 |
| المصدر المساهم: | HRCAK PORTAL ZNANSTVENIH CASOPISA REPUB From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1280900796 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |