مورد إلكتروني
A single-cell view on the intra- and inter-population metabolic heterogeneity and ecophysiology of microorganisms at different ecological scales
| العنوان: | A single-cell view on the intra- and inter-population metabolic heterogeneity and ecophysiology of microorganisms at different ecological scales |
|---|---|
| بيانات النشر: | 2021-02-02 2021-09-17 2021-11-04 |
| تفاصيل مُضافة: | Schlömann, Michael Harms, Hauke Musat, Niculina Technische Universität Bergakademie Freiberg Helmholtz Centre for Environmental Research - UFZ Calabrese, Federica |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Metabolic heterogeneity (MH) occurs when isogenic microbial populations display cell-to-cell differences in metabolic traits, albeit exposed to homogeneous conditions. Despite the increasing focus on MH, its triggering factors remain largely unknown. In the present thesis, I used stable isotope probing and chemical imaging with nanoscale Secondary Ion Mass Spectrometry (nanoSIMS) to study MH at single-cell level, in model organisms, synthetic and natural communities, to understand i) how abiotic factors, biotic interactions and antibiotics exposure influence MH and ii) its potential ecological role. Moreover, I optimized sample preparation for chemical and high-resolution imaging and suggested two different indices as ‘unit measure’ of MH. As results, I have shown for the first time that MH is displayed by microorganisms under favorable growth conditions, although none of the tested abiotic factors prevailed as the main trigger of MH. I brought insights on how biotic interactions play a role in the functional heterogeneity using bacteria pseudo-fungi co-cultures. I found that antibiotics reduce Carbon and Nitrogen assimilation rates of targeted phylogenetic groups in river-water communities, while increasing their MH, pointing to its ecological importance in natural environments. To conclude, I provided novel insights on the phenomenon of MH and its dynamics at different ecological scales.:Abbreviation list Summary Introduction Knowledge gaps Results and discussion - Optimization of sample preparation - Validation of quantitation methods - Abiotic factors shaping metabolic heterogeneity in bacterial populations - Influence of biotic factors in shaping heterogeneity - Metabolic Heterogeneity and ecophysiology of natural microbial populations influenced by emerging contaminants Conclusions Outlook Bibliography Appendix Acknowledgments Curriculum Vitae List of publications |
| مصطلحات الفهرس: | metabolic heterogeneity, single-cell analysis, stable isotope probing combined with nanoSIMS, heterogeneity quantitation, assimilation rates, Stoffwechselheterogenität, Einzelzellanalyse, stabile Isotope, nanoSIMS, Quantifizierung der Heterogenität, Assimilationsraten, info:eu-repo/classification/ddc/570, ddc:570, Mikroorganismus, Stoffwechsel, Einzelzellanalyse, Sekundärionen-Massenspektrometrie, Stabiles Isotop, Heterogenität, doc-type:doctoralThesis, info:eu-repo/semantics/doctoralThesis, doc-type:Text |
| URL: | 10.1111/1462-2920.15756 10.1116/1.5143203 10.3389/fmicb.2019.02814 10.3389/fmicb.2018.02342 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | English |
| أرقام أخرى: | SUUSL oai:qucosa:de:qucosa:76344 urn:nbn:de:bsz:105-qucosa2-763444 1285176293 |
| المصدر المساهم: | STAATS U UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1285176293 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |