مورد إلكتروني
Preliminary characterisation of digestive proteases of the green mirid, Creontiades dilutus (Hemiptera: Miridae)
| العنوان: | Preliminary characterisation of digestive proteases of the green mirid, Creontiades dilutus (Hemiptera: Miridae) |
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| المصدر: | Insect Biochemistry and Molecular Biology |
| بيانات النشر: | Pergamon-Elsevier Ltd 2001 |
| تفاصيل مُضافة: | Colebatch, Gillian East, Peter Cooper, Paul |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Protease activities in the secreted saliva, salivary glands and midgut of the green mirid, Creontiades dilutus, were investigated. The saliva and salivary glands had more protease activity than the midgut, but no differences in protease activity levels were detected between male and female mirids, adult mirids and third instar nymphs, or between fed and starved mirids. In the salivary glands, chymotrypsin-like serine proteases predominated, as characterised by inhibitor specificity, basic pH optima, and hydrolysis of N-benzoyl-L-tyrosine p-nitroanilide and N-succinyl-ala-ala-pro-leu p-nitroanilide. The pH optimum of midgut extracts was acidic (pH 4), implying that acidic proteases predominate. However, protease activity was inhibited substantially by both aprotinin and E-64, suggesting the presence of both serine and cysteine proteases in the midgut of the green mirid. |
| مصطلحات الفهرس: | Keywords: anilide; aprotinin; chymotrypsin; cysteine proteinase; intestine enzyme; n [n (3 carboxyoxirane 2 carbonyl)leucyl]agmatine; n benzoyltyrosine 4 nitroanilide; n succinylalanylalanylprolylleucine 4 nitroanilide; proteinase; serine proteinase; unclassified d Chymotrypsin; Gut; Heteroptera; Mirid; Protease; Salivary gland, Journal article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| أرقام أخرى: | AUANP oai:openresearch-repository.anu.edu.au:1885/91469 0965-1748 1291817746 |
| المصدر المساهم: | AUSTRALIAN NAT UNIV - PRINT REPOSITORY From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1291817746 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |