مورد إلكتروني
A Structural Study on the Rabies Virus Glycoprotein combining Cryo-EM and Mass Spectrometry-based Approaches
| العنوان: | A Structural Study on the Rabies Virus Glycoprotein combining Cryo-EM and Mass Spectrometry-based Approaches |
|---|---|
| المؤلفون: | Coelingh, Bob |
| بيانات النشر: | Utrecht University 2022 |
| تفاصيل مُضافة: | Snijder, J (Thesis Advisor) |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Rabies Virus (RABV) is a zoonotic neurotropic virus and the main causative agent for Rabies, a lethal encephalitis. The Rabies Virus Glycoprotein (RABV G) is a homo-trimer present on the viral envelope and is essential in the viral life cycle because of its role in receptor recognition and membrane fusion and is the main immunogenic target. Here we report an updated protocol for the purification of RABV G from cell supernatant, which was used for cryo-EM single particle analysis to elucidate its structure. Additionally we mapped the N-linked glycans of the protein and characterized the oligomerization using mass photometry, native MS and Charge-Detection MS, which hinted at a concentration-dependent equilibrium. We also assessed the binding of two therapeutically used neutralizing antibodies by HDX-MS and can point to a probable epitope on RABV G for both. |
| مصطلحات الفهرس: | The Rabies Virus (RABV) is a virus transmitted to humans via animals causing 60.000 deaths yearly. A very important protein in the life cycle of the Rabies Virus is the Glycoprotein (RABV G) which is essential for a successful infection. In this research we purified RABV G to obtain its structure, analyze its binding interactions with host receptors and neutralizing antibodies and characterize the occupation of sugar groups., M, Student Thesis |
| URL: | |
| الإتاحة: | Open access content. Open access content CC-BY-NC-ND Open Access |
| ملاحظة: | EN |
| أرقام أخرى: | QGJ oai:studenttheses.uu.nl:20.500.12932/454 https://studenttheses.uu.nl/handle/20.500.12932/454 1294447909 |
| المصدر المساهم: | UNIVERSITEIT UTRECHT From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1294447909 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |