مورد إلكتروني
Unexpected contribution of fibroblasts to muscle lineage as a mechanism for limb muscle patterning
| العنوان: | Unexpected contribution of fibroblasts to muscle lineage as a mechanism for limb muscle patterning |
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| المصدر: | Nature Communications vol.12 (2021) date: 2021-06-22 nr.1 p.3851 [ISSN 2041-1723] |
| بيانات النشر: | 2021 |
| تفاصيل مُضافة: | Esteves de Lima, Joana Blavet, Cédrine Bonnin, Marie-Ange Hirsinger, Estelle Comai, Glenda Yvernogeau, Laurent Delfini, Marie-Claire Bellenger, Léa Mella, Sébastien Nassari, Sonya Robin, Catherine Schweitzer, Ronen Fournier-Thibault, Claire Jaffredo, Thierry Tajbakhsh, Shahragim Relaix, Frédéric Duprez, Delphine |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Positional information driving limb muscle patterning is contained in connective tissue fibroblasts but not in myogenic cells. Limb muscles originate from somites, while connective tissues originate from lateral plate mesoderm. With cell and genetic lineage tracing we challenge this model and identify an unexpected contribution of lateral plate-derived fibroblasts to the myogenic lineage, preferentially at the myotendinous junction. Analysis of single-cell RNA-sequencing data from whole limbs at successive developmental stages identifies a population displaying a dual muscle and connective tissue signature. BMP signalling is active in this dual population and at the tendon/muscle interface. In vivo and in vitro gain- and loss-of-function experiments show that BMP signalling regulates a fibroblast-to-myoblast conversion. These results suggest a scenario in which BMP signalling converts a subset of lateral plate mesoderm-derived cells to a myogenic fate in order to create a boundary of fibroblast-derived myonuclei at the myotendinous junction that controls limb muscle patterning. |
| مصطلحات الفهرس: | Animals, Body Patterning/genetics, Cell Lineage/genetics, Cells, Cultured, Chick Embryo, Extremities/embryology, Fibroblasts/cytology, Gene Expression Regulation, Developmental, Mesoderm/cytology, Mice, Inbred C57BL, Mice, Inbred DBA, Mice, Transgenic, Muscle Development/genetics, Muscle, Skeletal/cytology, Reverse Transcriptase Polymerase Chain Reaction, Somites/cytology, Artikel, Article |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | DOI: 10.1038/s41467-021-24157-x Nature Communications vol.12 (2021) date: 2021-06-22 nr.1 p.3851 [ISSN 2041-1723] English |
| أرقام أخرى: | NLKNA oai:pure.knaw.nl:publications/281a36d2-3774-499a-9d68-6fd41682c1f4 https://pure.knaw.nl/portal/en/publications/281a36d2-3774-499a-9d68-6fd41682c1f4 1295315541 |
| المصدر المساهم: | KON NED AKADEMIE VAN WETENSCHAPPEN From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1295315541 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |