مورد إلكتروني
Interleukin-10 differentially modulates MHC class II expression by mesangial cells and macrophages in vitro and in vivo.
| العنوان: | Interleukin-10 differentially modulates MHC class II expression by mesangial cells and macrophages in vitro and in vivo. |
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| بيانات النشر: | Blackwell Publishing Ltd (9600 Garsington Road, Oxford OX4 2XG, United Kingdom) United Kingdom 2012-10-20 |
| تفاصيل مُضافة: | Chadban S.J. Atkins R.C. Tesch G.H. Lan H.Y. Foti R. Nikolic-Paterson D.J. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Inhibition of major histocompatibility complex (MHC) class II expression by macrophages is the primary mechanism by which interleukin-10 (IL-10) exerts immune suppression. Little, however, is known of the effects of IL-10 on other types of cells which can be induced to express MHC class II during an inflammatory response. We therefore studied the effects of IL-10 treatment on the expression of MHC class II molecules in a rat model of immunologically induced glomerulonephritis. MHC class II mRNA levels in whole kidney were increased in saline-treated (control) animals with glomerulonephritis (2-6- fold increase versus normal, P = 0.028) and this was partially inhibited by treatment with IL-10 (P=NS). Double immunostaining of tissue sections was used to compare MHC class II expression by infiltrating macrophages and resident glomerular cells. IL-10 treatment reduced the proportion of glomerular macrophages which expressed detectable MHC class II (70% reduction, P=0.03). In contrast, IL-10 treatment was associated with an increase in the number of resident glomerular cells expressing MHC class II, particularly within mesangial areas. Therefore, the effects of IL-10 on macrophages and mesangial cells were compared in vitro. IL-10 reduced constitutive MHC class II mRNA and cell surface expression by peritoneal macrophages. In contrast, IFN-7-stimulated mesangial cells (1097 cell line) cultured with IL-10 for 24 hr showed increased MHC class II mRNA (26% increase) and surface expression (72% increase in percentage MHC II+ by flow cytometry, P=0.04) as compared with cells stimulated with IFN-gamma alone. IL- 10 also directly up-regulated expression of ICAM-1 by 1097 cells. In conclusion, IL-10 was found to have contrasting effects on the production and cell surface expression of MHC class II molecules by mesangial cells and by macrophages, both in vitro and in vivo. The implications of these findings for IL-10 mediated immunosuppression are discussed. |
| مصطلحات الفهرس: | animal tissue, antigen expression, article, controlled study, glomerulonephritis, in vivo study, male, mesangium cell, nonhuman, animal cell, priority journal, rat, intercellular adhesion molecule 1/ec [Endogenous Compound], major histocompatibility antigen class 2/ec [Endogenous Compound], messenger RNA/ec [Endogenous Compound], neutralizing antibody, recombinant gamma interferon, recombinant interleukin 10, peritoneum macrophage, animal experiment, animal model, in vitro study, Article |
| URL: | Click here for full text options LibKey Link |
| الإتاحة: | Open access content. Open access content Copyright 2012 Elsevier B.V., All rights reserved. |
| أرقام أخرى: | AUSHL oai:repository.monashhealth.org:1/33797 Immunology. 94 (1) (pp 72-78), 1998. Date of Publication: 1998. 0019-2805 https://repository.monashhealth.org/monashhealthjspui/handle/1/33797 9708189 [http://www.ncbi.nlm.nih.gov/pubmed/?term=9708189] 28412790 (Chadban, Tesch, Foti, Lan, Atkins, Nikolic-Paterson) Department of Nephrology, Monash Medical Centre, Clayton, Vic., Australia (Chadban) Department of Nephrology, Monash Medical Centre, 246 Clayton Road, Clayton, Vic. 3168, Australia 1305130853 |
| المصدر المساهم: | MONASH HEALTH LIBRS From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1305130853 |
| قاعدة البيانات: | OAIster |
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