مورد إلكتروني
Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids
| العنوان: | Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids |
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| بيانات النشر: | Umeå universitet, Institutionen för molekylärbiologi (Medicinska fakulteten) Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary; Doctoral School of Biology, University of Szeged, Szeged, Hungary Department of Anatomy Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary; Doctoral School of Biology, University of Szeged, Szeged, Hungary Department of Anatomy Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Laboratory of Neural Connectivity, Brain Research Institute, University of Zurich, Zurich, Switzerland Laboratory of Neural Connectivity, Brain Research Institute, University of Zurich, Zurich, Switzerland Laboratory of Neural Connectivity, Brain Research Institute, University of Zurich, Zurich, Switzerland Laboratory of Bioinformatics, Biological Research Centre, Szeged, Hungary; Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Pécs, Hungary Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary Laboratory of Neural Connectivity, Brain Research Institute, University of Zurich, Zurich, Switzerland Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary 2022 |
| تفاصيل مُضافة: | Cinege, Gyöngyi Magyar, Lilla B. Kovács, Attila L. Lerner, Zita Juhász, Gábor Lukacsovich, David Winterer, Jochen Lukacsovich, Tamás Hegedus, Zoltán Kurucz, Éva Hultmark, Dan Földy, Csaba Andó, István |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Multinucleated giant hemocytes (MGHs) represent a novel type of blood cell in insects that participate in a highly efficient immune response against parasitoid wasps involving isolation and killing of the parasite. Previously, we showed that circulating MGHs have high motility and the interaction with the parasitoid rapidly triggers encapsulation. However, structural and molecular mechanisms behind these processes remained elusive. Here, we used detailed ultrastructural analysis and live cell imaging of MGHs to study encapsulation in Drosophila ananassae after parasitoid wasp infection. We found dynamic structural changes, mainly driven by the formation of diverse vesicular systems and newly developed complex intracytoplasmic membrane structures, and abundant generation of giant cell exosomes in MGHs. In addition, we used RNA sequencing to study the transcriptomic profile of MGHs and activated plasmatocytes 72 h after infection, as well as the uninduced blood cells. This revealed that differentiation of MGHs was accompanied by broad changes in gene expression. Consistent with the observed structural changes, transcripts related to vesicular function, cytoskeletal organization, and adhesion were enriched in MGHs. In addition, several orphan genes encoding for hemolysin-like proteins, pore-forming toxins of prokaryotic origin, were expressed at high level, which may be important for parasitoid elimination. Our results reveal coordinated molecular and structural changes in the course of MGH differentiation and parasitoid encapsulation, providing a mechanistic model for a powerful innate immune response. |
| مصطلحات الفهرس: | Drosophila, Encapsulation, Innate immunity, Multinucleated giant hemocyte, Transcriptome, Immunology, Immunologi, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1159.000520110 |
| URL: | Journal of Innate Immunity, 1662-811X, 2022, 14:4, s. 335-354 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | UPE oai:DiVA.org:umu-190827 doi:10.1159/000520110 PMID 34864742 ISI:000729135100001 Scopus 2-s2.0-85121134254 1306163923 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1306163923 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1159.000520110 |
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