مورد إلكتروني
VERSO:A COMPREHENSIVE FRAMEWORK FOR THE INFERENCE OF ROBUST PHYLOGENIES AND THE QUANTIFICATION OF INTRA-HOST GENOMIC DIVERSITY OF VIRAL SAMPLES
| العنوان: | VERSO:A COMPREHENSIVE FRAMEWORK FOR THE INFERENCE OF ROBUST PHYLOGENIES AND THE QUANTIFICATION OF INTRA-HOST GENOMIC DIVERSITY OF VIRAL SAMPLES |
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| المؤلفون: | Ramazzotti, Daniele |
| بيانات النشر: | 2020 |
| تفاصيل مُضافة: | Ramazzotti, D Angaroni, F Maspero, D Gambacorti-Passerini, C Antoniotti, M Graudenzi, A Piazza, R Ramazzotti, Daniele Angaroni, Fabrizio Maspero, Davide Gambacorti-Passerini, Carlo Antoniotti, Marco Graudenzi, Alex Piazza, Rocco |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | A global cross-discipline effort is ongoing to characterize the evolution of SARS-CoV-2 virus and generate reliable epidemiological models of its diffusion. To this end, phylogenomic approaches leverage accumulating genomic mutations to track the evolutionary history of the virus and benefit from the surge of sequences deposited in public databases. Yet, such methods typically rely on consensus sequences representing the dominant virus lineage, whereas a complex intra-host genomic composition is often observed within single hosts. Furthermore, most approaches might produce inaccurate results with noisy data and sampling limitations, as witnessed in most countries affected by the epidemics. We introduce VERSO (Viral Evolution ReconStructiOn), a new comprehensive framework for the characterization of viral evolution and transmission from sequencing data of viral genomes. Our probabilistic approach first delivers robust phylogenetic models from clonal variant profiles and then exploits variant frequency patterns to characterize and visualize the intra-host genomic diversity of samples, which may reveal uncovered infection events. We prove via extensive simulations that VERSO outperforms the state-of-the-art tools for phylogenetic inference, also in condition of noisy observations and sampling limitations. The application of our approach to 3960 SARS-CoV-2 samples from Amplicon sequencing and to 2766 samples from RNA-sequencing unravels robust phylogenomic models, improving the current knowledge on SARS-CoV-2 evolution and spread. Importantly, by exploiting co-occurrence patterns of minor variants, VERSO allows us to reveal uncovered infection paths, which are validated with contact tracing data. Moreover, the in-depth analysis of the mutational landscape of SARS-CoV-2 confirms a statistically significant increase of genomic diversity in time and allows us to identify a number of variants that are transiting from minor to clonal state in the population, as well as sever |
| مصطلحات الفهرس: | Phylogeny, Viral Sampling, SARS-CoV-2, info:eu-repo/semantics/other |
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| الإتاحة: | Open access content. Open access content |
| ملاحظة: | ELETTRONICO English |
| أرقام أخرى: | ITBAO oai:boa.unimib.it:10281/288883 10.1101/2020.04.22.044404 1311401128 |
| المصدر المساهم: | BICOCCA OPEN ARCH From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1311401128 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |