مورد إلكتروني
Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I
| العنوان: | Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I |
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| بيانات النشر: | Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi Uppsala universitet, Science for Life Laboratory, SciLifeLab Karolinska Inst, Translat Res Ctr Karolinska TRACK, Dept Lab Med, SE-14186 Stockholm, Sweden.;Karolinska Univ Hosp, Ctr Rare Dis, SE-17176 Stockholm, Sweden. MDPI AG 2022 |
| تفاصيل مُضافة: | Bergquist, Helen Inturi, Raviteja Zain, Rula Punga, Tanel |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | RNA molecules can adopt specific RNA triplex structures to execute critical biological functions. Human adenoviruses (HAdVs) are abundant pathogens encoding the essential, noncoding virus-associated RNA I (VA RNAI). Here, we employ a triplex-specific probing assay, based on the intercalating and cleaving agent benzoquinoquinoxaline 1, 10-phenanthroline (BQQ-OP), to unravel a potential RNA triplex formation in VA RNAI. The BQQ-OP cleavage of the pathogenic HAdV type 4 (HAdV-4) VA RNAI indicates that a potential triplex is formed involving the highly conserved stem 4 of the central domain and side stem 7. Further, the integrity of the HAdV-4 VA RNAI side stem 7 contributes to a potential triplex formation in vitro and virus growth in vivo. Collectively, we propose that the HAdV-4 VA RNAI can potentially form a biologically relevant triplex structure. |
| مصطلحات الفهرس: | adenovirus, VA RNAI, triplex RNA, BQQ-OP, Biochemistry and Molecular Biology, Biokemi och molekylärbiologi, Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy), Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci), Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.3390.ijms23063103 |
| URL: | International Journal of Molecular Sciences, 1661-6596, 2022, 23:6 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | UPE oai:DiVA.org:uu-472755 0000-0002-0561-367x doi:10.3390/ijms23063103 PMID 35328524 ISI:000775281400001 1312837657 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1312837657 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.3390.ijms23063103 |
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