مورد إلكتروني
Clinical ApoA-IV amyloid is associated with fibrillogenic signal sequence
| العنوان: | Clinical ApoA-IV amyloid is associated with fibrillogenic signal sequence |
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| بيانات النشر: | Uppsala universitet, Klinisk och experimentell patologi UCL, Ctr Amyloidosis & Acute Phase Prot, Wolfson Drug Discovery Unit, Div Med, London, England. UCL, Ctr Amyloidosis & Acute Phase Prot, Wolfson Drug Discovery Unit, Div Med, London, England. UCL, Ctr Amyloidosis & Acute Phase Prot, Wolfson Drug Discovery Unit, Div Med, London, England. UCL, Natl Amyloidosis Ctr, London, England.;Royal Free Hosp, London, England. UCL, Natl Amyloidosis Ctr, London, England.;Royal Free Hosp, London, England. UCL, Natl Amyloidosis Ctr, London, England.;Royal Free Hosp, London, England. UCL, Natl Amyloidosis Ctr, London, England.;Royal Free Hosp, London, England. Univ Pavia, Dept Mol Med Inst Biochem, Pavia, Italy. UCL, Ctr Amyloidosis & Acute Phase Prot, Wolfson Drug Discovery Unit, Div Med, London, England.;Univ Pavia, Dept Mol Med Inst Biochem, Pavia, Italy. Univ Udine, Dept Med DAME, Udine, Italy. UCL, Ctr Amyloidosis & Acute Phase Prot, Wolfson Drug Discovery Unit, Div Med, London, England. Univ Pavia, Dept Mol Med Inst Biochem, Pavia, Italy. Univ Pavia, Dept Mol Med Inst Biochem, Pavia, Italy. UCL, Natl Amyloidosis Ctr, London, England.;Royal Free Hosp, London, England. UCL, Natl Amyloidosis Ctr, London, England.;Royal Free Hosp, London, England. UCL, Ctr Amyloidosis & Acute Phase Prot, Wolfson Drug Discovery Unit, Div Med, London, England.;Univ Pavia, Dept Mol Med Inst Biochem, Pavia, Italy. UCL, Ctr Amyloidosis & Acute Phase Prot, Wolfson Drug Discovery Unit, Div Med, London, England. WILEY 2021 |
| تفاصيل مُضافة: | Canetti, Diana Nocerino, Paola Rendell, Nigel B. Botcher, Nicola Gilbertson, Janet A. Blanco, Angel Rowczenio, Dorota Morelli, Alessandra Mangione, P. Patrizia Corazza, Alessandra Verona, Guglielmo Giorgetti, Sofia Marchese, Loredana Westermark, Per Hawkins, Philip N. Gillmore, Julian D. Bellotti, Vittorio Taylor, Graham W. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Apolipoprotein A-IV amyloidosis is an uncommon form of the disease normally resulting in renal and cardiac dysfunction. ApoA-IV amyloidosis was identified in 16 patients attending the National Amyloidosis Centre and in eight clinical samples received for histology review. Unexpectedly, proteomics identified the presence of ApoA-IV signal sequence residues (p.18-43 to p.20-43) in 16/24 trypsin-digested amyloid deposits but in only 1/266 non-ApoA-IV amyloid samples examined. These additional signal residues were also detected in the cardiac sample from the Swedish patient in which ApoA-IV amyloid was first described, and in plasma from a single cardiac ApoA-IV amyloidosis patient. The most common signal-containing peptide observed in ApoA-IV amyloid, p.20-43, and to a far lesser extent the N-terminal peptide, p.21-43, were fibrillogenic in vitro at physiological pH, generating Congo red-positive fibrils. The addition of a single signal-derived alanine residue to the N-terminus has resulted in markedly increased fibrillogenesis. If this effect translates to the mature circulating protein in vivo, then the presence of signal may result in preferential deposition as amyloid, perhaps acting as seed for the main circulating native form of the protein; it may also influence other ApoA-IV-associated pathologies. (c) 2021 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland. |
| مصطلحات الفهرس: | ApoA-IV amyloidosis, ApoA-IV sequence coverage, ApoA-IV signal-containing peptide, amyloid proteomics, targeted mass spectrometry in serum, fibrillogenic ApoA-IV signal-containing peptide, Cell and Molecular Biology, Cell- och molekylärbiologi, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1002.path.5770 |
| URL: | Journal of Pathology, 0022-3417, 2021, 255:3, s. 311-318 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:uu-469861 0000-0001-5292-8124 0000-0002-3511-6789 0000-0001-9438-6511 0000-0002-2756-4995 0000-0002-9672-8463 doi:10.1002/path.5770 PMID 34331462 ISI:000689701500001 1312846551 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1312846551 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1002.path.5770 |
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