مورد إلكتروني
Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts
| العنوان: | Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts |
|---|---|
| المصدر: | International Journal of Molecular Sciences |
| بيانات النشر: | MDPI, Basel 2021 |
| تفاصيل مُضافة: | Simonović Radosavljević, Jasna Mitrović, Aleksandra Radotić, Ksenija Zimanyi, Laszlo Garab, Gyozo Steinbach, Gabor |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarization spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies. |
| مصطلحات الفهرس: | RCM, polarized light, molecular organization, micro-spectropolarimetry, fluorescence-detected linear dichroism, DP-LSM, circular dichroism, chloroplast, cell wall, anisotropy, article |
| URL: | Hungarian Ministry for National Economy (Economic Development and Innovation Operational Programme) [GINOP-2.3.3-15-201600003, GINOP-2.3.3-15-2016-00030, GINOP-2.1.7-15-2016-00713] info:eu-repo/grantAgreement/MESTD/inst-2020/200053/RS National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA K 128679] GINOP-2.3.2-15-2016-00001] |
| الإتاحة: | Open access content. Open access content https://creativecommons.org/licenses/by/4.0 BY openAccess |
| ملاحظة: | International Journal of Molecular Sciences |
| أرقام أخرى: | RSPST oai:rimsi.imsi.bg.ac.rs:123456789/1427 1422-0067 10.3390/ijms22147661 34299279 2-s2.0-85110246274 000676684200001 1314835820 |
| المصدر المساهم: | UIVERSITY OF BELGRADE From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1314835820 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |