مورد إلكتروني
Increased Expression of Cytotoxic T-Lymphocyte-Associated Protein 4 by T Cells, Induced by B7 in Sera, Reduces Adaptive Immunity in Patients With Acute Liver Failure
| العنوان: | Increased Expression of Cytotoxic T-Lymphocyte-Associated Protein 4 by T Cells, Induced by B7 in Sera, Reduces Adaptive Immunity in Patients With Acute Liver Failure |
|---|---|
| المصدر: | Khamri , W , Abeles , R D , Hou , T Z , Anderson , A E , El-Masry , A , Triantafyllou , E , Bernsmeier , C , Larsen , F S , Singanayagam , A , Kudo , N , Possamai , L A , Lebosse , F , Auzinger , G , Bernal , W , Willars , C , Weston , C J , Lombardi , G , Wendon , J , Thursz , M & Antoniades , C G 2017 , ' Increased Expression of Cytotoxic T-Lymphocyte-Associated Protein 4 by T Cells, Induced by B7 in Sera, Reduces Adaptive Immunity in Patients With Acute Liver Failure ' , Gastroenterology , vol. 153 , no. 1 , pp. 263-276.e8 . |
| بيانات النشر: | 2017 |
| تفاصيل مُضافة: | Khamri, Wafa Abeles, Robin D Hou, Tie Zheng Anderson, Amy E El-Masry, Ahmed Triantafyllou, Evangelos Bernsmeier, Christine Larsen, Fin S Singanayagam, Arjuna Kudo, Nobuaki Possamai, Lucia A Lebosse, Fanny Auzinger, Georg Bernal, William Willars, Christopher Weston, Christopher J Lombardi, Giovanna Wendon, Julia Thursz, Mark Antoniades, Charalambos G |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | BACKGROUND & AIMS: Patients with acute liver failure (ALF) have defects in innate immune responses to microbes (immune paresis) and are susceptible to sepsis. Cytotoxic T-lymphocyte-associated protein 4 (CTLA4), which interacts with the membrane receptor B7 (also called CD80 and CD86), is a negative regulator of T-cell activation. We collected T cells from patients with ALF and investigated whether inhibitory signals down-regulate adaptive immune responses in patients with ALF.METHODS: We collected peripheral blood mononuclear cells from patients with ALF and controls from September 2013 through September 2015 (45 patients with ALF, 20 patients with acute-on-chronic liver failure, 15 patients with cirrhosis with no evidence of acute decompensation, 20 patients with septic shock but no cirrhosis or liver disease, and 20 healthy individuals). Circulating CD4+ T cells were isolated and analyzed by flow cytometry. CD4+ T cells were incubated with antigen, or agonist to CD3 and dendritic cells, with or without antibody against CTLA4; T-cell proliferation and protein expression were quantified. We measured levels of soluble B7 molecules in supernatants of isolated primary hepatocytes, hepatic sinusoidal endothelial cells, and biliary epithelial cells from healthy or diseased liver tissues. We also measured levels of soluble B7 serum samples from patients and controls, and mice with acetaminophen-induced liver injury using enzyme-linked immunosorbent assays.RESULTS: Peripheral blood samples from patients with ALF had a higher proportion of CD4+ CTLA4+ T cells than controls; patients with infections had the highest proportions. CD4+ T cells from patients with ALF had a reduced proliferative response to antigen or CD3 stimulation compared to cells from controls; incubation of CD4+ T cells from patients with ALF with an antibody against CTLA4 increased their proliferative response to antigen and to CD3 stimulation, to the same levels as cells from control |
| مصطلحات الفهرس: | Acetaminophen/toxicity, Acute-On-Chronic Liver Failure/immunology, Adaptive Immunity, Adult, Animals, Antibodies/pharmacology, B7-1 Antigen/blood, CD3 Complex/pharmacology, CD4 Lymphocyte Count, CD4-Positive T-Lymphocytes/immunology, CTLA-4 Antigen/immunology, Cell Proliferation, Cells, Cultured, Chemical and Drug Induced Liver Injury/blood, Coculture Techniques, Dendritic Cells, Hepatocytes/secretion, Humans, Liver Cirrhosis/immunology, Liver Failure, Acute/immunology, Lymphocyte Activation, Mice, Middle Aged, Shock, Septic/immunology, article |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | DAV oai:pure.atira.dk:publications/25c0eb5f-d438-47fb-a679-aba50ffb7221 1322711824 |
| المصدر المساهم: | UNIV OF COPENHAGEN From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1322711824 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |