مورد إلكتروني
The GAGOme:a cell-based library of displayed glycosaminoglycans
| العنوان: | The GAGOme:a cell-based library of displayed glycosaminoglycans |
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| المصدر: | Chen , Y-H , Narimatsu , Y , Clausen , T M , Gomes , C , Karlsson , R , Steentoft , C , Spliid , C B , Gustavsson , T , Salanti , A , Persson , A , Malmström , A , Willén , D , Ellervik , U , Bennett , E P , Mao , Y , Clausen , H & Yang , Z 2018 , ' The GAGOme : a cell-based library of displayed glycosaminoglycans ' , Nature Methods , vol. 15 , no. 11 , pp. 881-888 . |
| بيانات النشر: | 2018 |
| تفاصيل مُضافة: | Chen, Yen-Hsi Narimatsu, Yoshiki Clausen, Thomas Mandel Gomes, Catarina Karlsson, Richard Steentoft, Catharina Spliid, Charlotte Bredo Gustavsson, Tobias Salanti, Ali Persson, Andrea Malmström, Anders Willén, Daniel Ellervik, Ulf Bennett, Eric Paul Mao, Yang Clausen, Henrik Yang, Zhang |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Glycosaminoglycans (GAGs) are essential polysaccharides in normal physiology and disease. However, understanding of the contribution of specific GAG structures to specific biological functions is limited, largely because of the great structural heterogeneity among GAGs themselves, as well as technical limitations in the structural characterization and chemical synthesis of GAGs. Here we describe a cell-based method to produce and display distinct GAGs with a broad repertoire of modifications, a library we refer to as the GAGOme. By using precise gene editing, we engineered a large panel of Chinese hamster ovary cells with knockout or knock-in of the genes encoding most of the enzymes involved in GAG biosynthesis, to generate a library of isogenic cell lines that differentially display distinct GAG features. We show that this library can be used for cell-based binding assays, recombinant expression of proteoglycans with distinct GAG structures, and production of distinct GAG chains on metabolic primers that may be used for the assembly of GAG glycan microarrays. |
| مصطلحات الفهرس: | article |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/closedAccess |
| ملاحظة: | English |
| أرقام أخرى: | DAV oai:pure.atira.dk:publications/1a40647c-e39c-440f-ad98-062eed962229 1322714547 |
| المصدر المساهم: | UNIV OF COPENHAGEN From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1322714547 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |