مورد إلكتروني
PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules
| العنوان: | PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules |
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| المصدر: | Buchardt , O , Freier , S M , Driver , D A , Kim , S K , Norden , B & Nielsen , P E 1993 , ' PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules ' , Nature , vol. 365 , no. 6446 , pp. 566-8 . |
| بيانات النشر: | 1993-10-07 |
| تفاصيل مُضافة: | Buchardt, O Freier, S M Driver, D A Kim, S K Norden, B Nielsen, Peter E. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | DNA analogues are currently being intensely investigated owing to their potential as gene-targeted drugs. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to which the nucleobases are attached. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson-Crick-Hoogsteen (PNA)2-DNA triplexes, which are much more stable than the corresponding DNA-DNA duplexes, and bind to double-stranded DNA by strand displacement. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson-Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition. |
| مصطلحات الفهرس: | Base Sequence, Cytosine/chemistry, DNA/chemistry, Glycine/analogs & derivatives, Hydrogen Bonding, Molecular Sequence Data, Nucleic Acid Hybridization, Oligodeoxyribonucleotides/chemistry, RNA/chemistry, Thermodynamics, Thymine/chemistry, article |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/closedAccess |
| ملاحظة: | English |
| أرقام أخرى: | DAV oai:pure.atira.dk:publications/766ed032-2bda-4df7-9517-6db73b038cac 1322716961 |
| المصدر المساهم: | UNIV OF COPENHAGEN From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1322716961 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |