مورد إلكتروني
Probing skin barrier recovery on molecular level following acute wounds:An in vivo/ex vivo study on pigs
| العنوان: | Probing skin barrier recovery on molecular level following acute wounds:An in vivo/ex vivo study on pigs |
|---|---|
| المصدر: | Mojumdar , E H , Madsen , L B , Hansson , H , Taavoniku , I , Kristensen , K , Persson , C , Morén , A K , Mokso , R , Schmidtchen , A , Ruzgas , T & Engblom , J 2021 , ' Probing skin barrier recovery on molecular level following acute wounds : An in vivo/ex vivo study on pigs ' , Biomedicines , vol. 9 , no. 4 , 360 . |
| بيانات النشر: | 2021 |
| تفاصيل مُضافة: | Mojumdar, Enamul Haque Madsen, Lone Bruhn Hansson, Henri Taavoniku, Ida Kristensen, Klaus Persson, Christina Morén, Anna Karin Mokso, Rajmund Schmidtchen, Artur Ruzgas, Tautgirdas Engblom, Johan |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Proper skin barrier function is paramount for our survival, and, suffering injury, there is an acute need to restore the lost barrier and prevent development of a chronic wound. We hypothesize that rapid wound closure is more important than immediate perfection of the barrier, whereas specific treatment may facilitate perfection. The aim of the current project was therefore to evaluate the quality of restored tissue down to the molecular level. We used Göttingen minipigs with a multi-technique approach correlating wound healing progression in vivo over three weeks, monitored by classical methods (e.g., histology, trans-epidermal water loss (TEWL), pH) and subsequent physicochemical characterization of barrier recovery (i.e., small and wide-angle X-ray diffraction (SWAXD), polarization transfer solid-state NMR (PTssNMR), dynamic vapor sorption (DVS), Fourier transform infrared (FTIR)), providing a unique insight into molecular aspects of healing. We conclude that although acute wounds sealed within two weeks as expected, molecular investigation of stratum corneum (SC) revealed a poorly developed keratin organization and deviations in lipid lamellae formation. A higher lipid fluidity was also observed in regenerated tissue. This may have been due to incomplete lipid conversion during barrier recovery as glycosphingolipids, normally not present in SC, were indicated by infrared FTIR spectroscopy. Evidently, a molecular approach to skin barrier recovery could be a valuable tool in future development of products targeting wound healing. |
| مصطلحات الفهرس: | Acute wound, Histology, In vivo/ex vivo, Lipid, PH, Polarization transfer solid state NMR (PTssNMR), Skin barrier, Small and wide-angle X-ray diffraction (SWAXD), Stratum corneum, Trans-epidermal water loss (TEWL), article |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | DAV oai:pure.atira.dk:publications/93fd02dd-271c-423e-8aaa-ff57d3399c31 1322766783 |
| المصدر المساهم: | UNIV OF COPENHAGEN From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1322766783 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |