مورد إلكتروني
C/EBPβ Promotes LPS-Induced IL-1β Transcription and Secretion in Alveolar Macrophages via NOD2 Signaling
| العنوان: | C/EBPβ Promotes LPS-Induced IL-1β Transcription and Secretion in Alveolar Macrophages via NOD2 Signaling |
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| المؤلفون: | Luo,Yalan, Ge,Peng, Wen,Haiyun, Zhang,Yibo, Liu,Jin, Dong,Xuanchi, Lan,Bowen, Zhang,Guixin, Yang,Qi, Chen,Hailong |
| بيانات النشر: | Dove Press 2022-09-11 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Yalan Luo,1– 3,* Peng Ge,1– 3,* Haiyun Wen,1– 3,* Yibo Zhang,1– 3 Jin Liu,1,3 Xuanchi Dong,1,3 Bowen Lan,1,3 Guixin Zhang,1– 3 Qi Yang,4 Hailong Chen1– 3 1Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, People’s Republic of China; 2Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, People’s Republic of China; 3Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, People’s Republic of China; 4Department of Traditional Chinese Medicine, The Second Affiliated Hospital of Dalian Medical University, Dalian, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qi Yang; Hailong Chen, Email dyeyyq1016@163.com; chenhailong@dmu.edu.cnObjective: C/EBPβ, a crucial transcription factor, regulates innate immunity and inflammatory responses. However, the role played by C/EBPβ in alveolar macrophage (AM) inflammatory responses remains unknown. This study aimed to investigate the role and mechanism of C/EBPβ in alveolar macrophages (AMs) from the transcriptional level and to search for natural compounds targeting C/EBPβ.Methods: Rat AMs were infected with Lv-sh-C/EBPβ and treated with LPS, and the expression levels of iNOS, TNF-α, IL-6, and IL-1β were measured by RT-qPCR, Western blotting, and ELISA. Mechanistically, transcriptome sequencing (RNA-seq) revealed changes in gene expression patterns in AMs after LPS stimulation and C/EBPβ knockdown. Functional enrichment analyses and rescue experiments identified and validated inflammation-associated cell signaling pathways regulated by C/EBPβ. Furthermore, virtual screening was used to search for natural compounds that inhibit C/EBPβ with the structure of helenalin as a reference.Resul |
| مصطلحات الفهرس: | Journal of Inflammation Research, Original Research, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/doi/10.2147/JIR.S377499 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | text/html English |
| أرقام أخرى: | NZDMP oai:dovepress.com/78128 1351715383 |
| المصدر المساهم: | DOVE MEDL PRESS LTD From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1351715383 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |