مورد إلكتروني
Self-assembling VHH-elastin-like peptides for photodynamic nanomedicine
| العنوان: | Self-assembling VHH-elastin-like peptides for photodynamic nanomedicine |
|---|---|
| المصدر: | Biomacromolecules vol.18 (2017) date: 2017-04-01 nr.4 p.1302-1310 [ISSN 1525-7797] |
| بيانات النشر: | 2017 |
| تفاصيل مُضافة: | Pille, J. Pille, J. van Lith, S.A.M. van Hest, J.C.M. Leenders, W.P.J. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Recombinant llama heavy-chain antibody fragments (VHHs) are promising tools in the field of targeted nanomedicine. 7D12, a VHH against the epidermal growth factor receptor (EGFR) that is overexpressed in various cancers, has been evaluated as an effective cancer-targeting VHH in multiple studies. The small size of VHHs (15-20 kDa) results in a low circulation half-life, which can be disadvantageous for certain applications. A solution to this problem is to attach VHHs to the surface of nanoparticles to increase the hydrodynamic radius of the conjugate. This approach simultaneously allows the incorporation of different VHHs and other targeting moieties and therapeutic components into one structure, creating multispecificity and versatility for therapy and diagnosis. Here, we present the construction of highly defined 7D12-containing nanoparticles by utilizing thermoresponsive diblock elastin-like peptides that reversibly self-assemble into micellar structures. The resulting particles have a hydrodynamic radius of 24.3 +/- 0.9 rim and retain full EGFR-binding capacity. We present proof of concept of the usability of such particles by controlled incorporation of a photosensitizer and show that the resulting nanoparticles induce EGFR-specific light-induced cell killing. This approach is easily extended to the controlled incorporation of various functional modules, improving therapy and diagnosis with targeted nanomedicine. |
| مصطلحات الفهرس: | Animals, Camelids, New World, Cell Line, Tumor, Cell Survival/drug effects, Drug Stability, Elastin/chemistry, ErbB Receptors/antagonists & inhibitors, Escherichia coli/genetics, Humans, Light, Nanomedicine, Nanoparticles/chemistry, Peptides/chemistry, Photochemotherapy, Photosensitizing Agents/chemistry, Recombinant Fusion Proteins/blood, Single-Domain Antibodies/chemistry, SDG 3 - Good Health and Well-being, Tijdschriftartikel, Article |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | DOI: 10.1021/acs.biomac.7b00064 Biomacromolecules vol.18 (2017) date: 2017-04-01 nr.4 p.1302-1310 [ISSN 1525-7797] English |
| أرقام أخرى: | NLTUR oai:pure.tue.nl:publications/c5089059-c640-4df4-897d-3f03dff5d392 https://research.tue.nl/en/publications/c5089059-c640-4df4-897d-3f03dff5d392 1359179739 |
| المصدر المساهم: | TU/E REPOSITORY From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1359179739 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |