مورد إلكتروني
TUB gene expression in hypothalamus and adipose tissue and its association with obesity in humans
| العنوان: | TUB gene expression in hypothalamus and adipose tissue and its association with obesity in humans |
|---|---|
| المؤلفون: | Nies, V. J. M. |
| المصدر: | International Journal of Obesity vol.42 (2018) nr.3 p.376-383 [ISSN 0307-0565] |
| بيانات النشر: | 2018 |
| تفاصيل مُضافة: | Struik, D. Wolfs, M. G. M. Rensen, S. S. Szalowska, E. Unmehopa, U. A. Fluiter, K. van der Meer, T. P. Hajmousa, G. Buurman, W. A. Greve, J. W. Rezaee, F. Shiri-Sverdlov, R. Vonk, R. J. Swaab, D. F. Wolffenbuttel, B. H. R. Jonker, J. W. van Vliet-Ostaptchouk, J. V. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | BACKGROUND/OBJECTIVES: Mutations in the Tubby gene (TUB) cause late-onset obesity and insulin resistance in mice and syndromic obesity in humans. Although TUB gene function has not yet been fully elucidated, studies in rodents indicate that TUB is involved in the hypothalamic pathways regulating food intake and adiposity. Aside from the function in central nervous system, TUB has also been implicated in energy metabolism in adipose tissue in rodents. We aimed to determine the expression and distribution patterns of TUB in man as well as its potential association with obesity.SUBJECTS/METHODS: In situ hybridization was used to localize the hypothalamic regions and cells expressing TUB mRNA. Using RT-PCR, we determined the mRNA expression level of the two TUB gene alternative splicing isoforms, the short and the long transcript variants, in the hypothalami of 12 obese and 12 normal-weight subjects, and in biopsies from visceral (VAT) and subcutaneous (SAT) adipose tissues from 53 severely obese and 24 non-obese control subjects, and correlated TUB expression with parameters of obesity and metabolic health.RESULTS: Expression of both TUB transcripts was detected in the hypothalamus, whereas only the short TUB isoform was found in both VAT and SAT. TUB mRNA was detected in several hypothalamic regions involved in body weight regulation, including the nucleus basalis of Meynert and the paraventricular, supraoptic and tuberomammillary nuclei. We found no difference in the hypothalamic TUB expression between obese and control groups, whereas the level of TUB mRNA was significantly lower in adipose tissue of obese subjects as compared to controls. Also, TUB expression was negatively correlated with indices of body weight and obesity in a fat-depot-specific manner.CONCLUSIONS: Our results indicate high expression of TUB in the hypothalamus, especially in areas involved in body weight regulation, and the correlation between TUB expression in adipose ti |
| مصطلحات الفهرس: | CANDIDATE GENE, ARCUATE NUCLEUS, NEUROPEPTIDE-Y, MESSENGER-RNA, TUB/TUB MICE, BODY-WEIGHT, PROTEINS, MUTATION, MOUSE, INSULIN, Article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | DOI: 10.1038/ijo.2017.214 English |
| أرقام أخرى: | NLMAT oai:cris.maastrichtuniversity.nl:publications/1fcb5e24-9b17-4e0b-ab5a-ef01cc74941b https://cris.maastrichtuniversity.nl/en/publications/1fcb5e24-9b17-4e0b-ab5a-ef01cc74941b 1363318142 |
| المصدر المساهم: | UNIVERSITEIT MAASTRICHT From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363318142 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |