مورد إلكتروني
Functional restoration of cultured mouse pancreatic islets after in vitro exposure to alloxan.
| العنوان: | Functional restoration of cultured mouse pancreatic islets after in vitro exposure to alloxan. |
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| المصدر: | Pharmacology & toxicology, 63 (5 |
| بيانات النشر: | 1988 |
| تفاصيل مُضافة: | Eizirik, Decio L. Sandler, S |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The molecular mechanisms behind the functional responses of the beta-cells after cytotoxic damage are still largely unknown. The aim of this study was to investigate to what extent the islet beta-cells are capable of repairing cellular injuries after acute treatment with increasing doses of alloxan. Isolated mouse pancreatic islets were exposed for 30 min. at 37 degrees to alloxan (1.0, 1.5 and 2.0 mM) or vehicle alone (controls). Immediately after alloxan exposure the islet glucose-stimulated insulin release was severely decreased, and there were morphological evidences of partial necrosis of the islets. After further six days in culture, there was a marked decrease in islet number in the groups of islets treated with 1.5 or 2.0 mM alloxan. However, the DNA and insulin contents of the remaining islets were similar to the values observed in cultured control islets. Furthermore, the insulin secretory response to glucose and the light microscopical appearance of these islets were largely restored on day 6. It is concluded that beta-cells surviving after an injury induced by alloxan may recover their functional capacity after an initial period of inhibited function. Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Alloxan -- pharmacology, Alloxan -- toxicity, Animals, Cell Survival, Cells, Cultured, DNA -- analysis, Insulin -- secretion, Islets of Langerhans -- drug effects, Islets of Langerhans -- physiology, Mice, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/55960 uri/info:pmid/3070521 1363711063 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363711063 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |