مورد إلكتروني
Allosteric transinhibition by specific antagonists in CCR2/CXCR4 heterodimers.
| العنوان: | Allosteric transinhibition by specific antagonists in CCR2/CXCR4 heterodimers. |
|---|---|
| المصدر: | The Journal of biological chemistry, 282 (41 |
| بيانات النشر: | 2007-10 |
| تفاصيل مُضافة: | Sohy, Denis Parmentier, Marc Springael, Jean-Yves |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Chemokine receptors are presently used as targets for candidate drugs in the frame of inflammatory diseases and human immunodeficiency virus infection. They were shown to dimerize, but the functional relevance of dimerization in terms of drug action remains poorly understood. We reported previously the existence of negative binding cooperativity between the subunits of CCR2/CCR5 heterodimers. In the present study, we extend these observations to heterodimers formed by CCR2 and CXCR4, which are more distantly related. We also show that specific antagonists of one receptor inhibit the binding of chemokines to the other receptor as a consequence of their heterodimerization, both in recombinant cell lines and primary leukocytes. This resulted in a significant functional cross-inhibition in terms of calcium mobilization and chemotaxis. These data demonstrate that chemokine receptor antagonists regulate allosterically the functional properties of receptors on which they do not bind directly, with important implications on the effects of these potential therapeutic agents. Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Allosteric Site, Animals, Binding, Competitive, CHO Cells, Calcium -- metabolism, Chemotaxis, Cricetinae, Cricetulus, Dimerization, Humans, Inhibitory Concentration 50, Kinetics, Protein Binding, Receptors, CCR2 -- chemistry, Receptors, CXCR4 -- chemistry, T-Lymphocytes -- metabolism, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 1 full-text file(s): application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/51668 uri/info:doi/10.1074/jbc.M705302200 uri/info:pii/M705302200 uri/info:pmid/17715128 uri/info:scp/35648971157 uri/info:ulb-repo/set/1 1363712730 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363712730 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |