مورد إلكتروني
Upregulation of galectins-1 and -3 in human colon cancer and their role in regulating cell migration.
| العنوان: | Upregulation of galectins-1 and -3 in human colon cancer and their role in regulating cell migration. |
|---|---|
| المصدر: | International journal of cancer, 103 (3 |
| بيانات النشر: | 2003-01 |
| تفاصيل مُضافة: | Hittelet, Axel-Benoit Legendre, Hugues Nagy, Nathalie Bronckart, Yves Pector, Jean Claude Salmon, Isabelle Yeaton, Paul Gabius, Hans-Joachim Kiss, Robert Camby, Isabelle |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | To probe the potential contribution of beta-galactoside-contributing epitopes and receptor proteins (gal-1 and gal-3) to colon malignancy, we first examined the expression of galectins and binding sites in clinical specimens by lectin and immunohistochemistry. Sixty-seven colonic surgical resections were studied, including 10 normal, 10 mild dysplasias, 10 severe dysplasias and 37 cancers. gal-1 and gal-3 were expressed in variable amounts in the epithelial cells and the connective tissue of normal colon. Their expression significantly increased with the degree of dysplasia, suggesting that gal-1 and gal-3 and their binding sites are related to malignant progression, while gal-8 has been associated with suppressor activity. To study the functional aspects, the influence of these galectins on the migration of 4 human colorectal cancer cell lines (HCT-15, LoVo, DLD-1, CoLo201) was studied. In agreement with histopathologic monitoring, these tumor cells were found to produce gal-3, while only CoLo201 was positive for gal-1. Except for DLD-1 and gal-1, the lines exhibited gal-1 binding sites on the surface, prompting study by computer-assisted videomicroscopy of the effect on cell migration of the presence of galectin on the culture substrate. The level of cell migration for HCT-15, LoVo and CoLo201 cells was significantly reduced by 0.15 microg/cm(2) gal-1, and the presence of a blocking antibody at least reduced this effect. gal-3 significantly reduced cell migration in all 4 of the in vitro cell lines. In Vitro Journal Article Research Support, Non-U.S. Gov't FLWIN info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Adenocarcinoma -- metabolism, Adenocarcinoma -- pathology, Adenoma -- metabolism, Adenoma -- pathology, Animals, Cell Movement, Colonic Neoplasms -- metabolism, Colonic Neoplasms -- pathology, DNA Primers -- chemistry, Galectin 1 -- genetics, Galectin 1 -- metabolism, Galectin 2 -- genetics, Galectin 2 -- metabolism, Humans, Immunoenzyme Techniques, Lectins, Mice, Mice, Nude, Microscopy, Fluorescence, Microscopy, Phase-Contrast, Neoplasm Staging, Neoplasm Transplantation, Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured -- metabolism, Tumor Cells, Cultured -- pathology, Tumor Markers, Biological -- metabolism, Up-Regulation, Cell migration, Colon cancer, Dysplasia, Galectin-1, Galectin-3, In vitro cell line, In vivo xenograft, Integrin, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 1 full-text file(s): application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/52180 uri/info:doi/10.1002/ijc.10843 uri/info:pmid/12471620 uri/info:scp/0037454758 1363713197 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363713197 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |