مورد إلكتروني
Inhibition of basolateral cAMP permeability in the toad urinary bladder
| العنوان: | Inhibition of basolateral cAMP permeability in the toad urinary bladder |
|---|---|
| المصدر: | Journal of physiology, 528 (1 |
| بيانات النشر: | 2000-10 |
| تفاصيل مُضافة: | Boom, Alain Golstein, Philippe Frerotte, M Sande, J V Beauwens, Renaud |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | 1. The effect of sulphonylurea drugs on hydrosmotic flow across toad urinary bladder epithelium was re-evaluated in the present study. Glibenclamide, added to the basolateral medium, significantly enhanced the osmotic flow induced by low doses of antidiuretic hormone (ADH) or forskolin (FK), while it inhibited the effect of exogenous cyclic adenosine monophosphate (cAMP) or its non-hydrolysable bromo derivative, 8-Br-cAMP, added to the basolateral medium. These opposite effects of glibenclamide on the transepithelial osmotic flow can be explained by a reduction of cAMP permeability across the basolateral membrane of the epithelium. The decrease in cAMP permeability leads, according to the direction of the cAMP gradient, to firstly an enhanced osmotic flow when cAMP is generated intracellularly by addition of ADH and FK, glibenclamide reducing cAMP exit from the cell, and secondly a decreased osmotic flow in response to cAMP (and 8-Br-cAMP) added to the basolateral medium, glibenclamide inhibiting, in this case, their entry into the cell. 2. The demonstration that glibenclamide actually inhibits the basolateral cAMP permeability rests on the fact that firstly it decreases the release of cAMP into the basolateral medium by about 40 %, at each concentration of ADH or forskolin tested, secondly it increases the cAMP content of paired hemibladders incubated in the presence of ADH or FK, when intracellular degradation was prevented by phosphodiesterase inhibition, and thirdly it decreases also the uptake of basolateral 8-Br-[3H]cAMP into paired toad hemibladders. 3. Taken together, the present data demonstrate that glibenclamide inhibits the toad urinary bladder basolateral membrane permeability to cAMP, most probably by a direct interaction with a membrane protein not yet indentified but distinct from the sulphonylurea receptor. In Vitro Journal Article Research Support, Non-U.S. Gov't FLWIN info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, 8-Bromo Cyclic Adenosine Monophosphate -- pharmacokinetics, 8-Bromo Cyclic Adenosine Monophosphate -- pharmacology, ATP-Binding Cassette Transporters, Animals, Anthranilic Acids -- pharmacology, Bufo marinus, Calcium Channel Blockers -- pharmacology, Cyclic AMP -- metabolism, Cyclic AMP -- pharmacology, Dose-Response Relationship, Drug, Epithelium -- drug effects, Epithelium -- metabolism, Forskolin -- pharmacology, Glyburide -- pharmacology, Membrane Fluidity -- physiology, Osmosis -- drug effects, Permeability -- drug effects, Pinacidil -- pharmacology, Potassium Channels -- drug effects, Potassium Channels -- metabolism, Potassium Channels, Inwardly Rectifying, Receptors, Drug -- drug effects, Receptors, Drug -- metabolism, Urinary Bladder -- metabolism, Vasodilator Agents -- pharmacology, Vasopressins -- pharmacology, Water -- metabolism, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 2 full-text file(s): info:eu-repo/semantics/restrictedAccess | info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 2 full-text file(s): application/pdf | application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/53001 uri/info:doi/10.1111/j.1469-7793.2000.t01-1-00189.x uri/info:pii/PHY_0982 uri/info:pmid/11018117 uri/info:pmcid/PMC2270122 1363723099 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363723099 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |