مورد إلكتروني
Similarities in the stimulus-secretion coupling mechanisms of glucose- and 2-keto acid-induced insulin release.
| العنوان: | Similarities in the stimulus-secretion coupling mechanisms of glucose- and 2-keto acid-induced insulin release. |
|---|---|
| المصدر: | Endocrinology, 106 (1 |
| بيانات النشر: | 1980-01 |
| تفاصيل مُضافة: | Hutton, J C Sener, Abdullah Herchuelz, André Atwater, I Kawazu, Shoji Boschero, Antonio Carlos Somers, G Devis, Ghislain Malaisse, Willy |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The stimulus-secretion coupling of 2-keto acid-induced insulin release was investigated using 2-ketoisocaproate (4-methyl-2-oxopentanoate) as the principal model secretagogue. 2-Ketoisocaproate and 2-ketocaproate (2-oxo-, hexanoate) provoked changes in B cell electrical behavior characterized by an initial depolarization of the membrane potential, followed by rapid spike activity, which appeared either in a bursting pattern or as continuous activity. The onset of spike activity induced by 2-ketoisocaproate (5 mM) was biphasic in nature. The dynamic pattern of 2-ketoisocaproate-induced insulin release was also biphasic. 2-[U-14C]Ketoisocaproate (10 mM) was oxidized in islet tissue at a rate equivalent to that of [U-14C]glucose (17 mM) and a t a higher rate than 2-ketoisovalerate (3-methyl-2-oxobutyrate) and 2-keto-3-methyl-valerate, which were poor secretagogues. Like glucose, 2-ketoisocaproate provoked characteristic changes in 86Rb and 45Ca efflux from prelabeled islets and stimulated 45Ca net uptake. Proinsulin synthesis was stimulated by 2-ketoisocaproate through both a general effect on protein synthesis and a specific effect on hormonal biosynthesis. 2-Ketoisocaproate and 2-ketocaproate reproduced the effect of glucose on the islet content of ATP, ADP, AMP, NAD+, NADH, NADP+, and NADPH. These findings together with a series of observations on the effects upon the above parameters of site-specific inhibitors, e.g. respiratory inhibitors, suloctidil, theophylline, and epinephrine, suggested that the stimulus-secretion-coupling mechanisms for 2-ketoisocaproate- and glucose-induced release are similar. It is postulated that glucose- and 2-keto acid-induced insulin release may be initiated by a common signal. Journal Article info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Adenine Nucleotides -- metabolism, Animals, Biological Transport -- drug effects, Calcium -- metabolism, Female, Glucose -- metabolism, Glucose -- pharmacology, Insulin -- secretion, Islets of Langerhans -- drug effects, Islets of Langerhans -- physiology, Keto Acids -- metabolism, Keto Acids -- pharmacology, Kinetics, Membrane Potentials -- drug effects, Oxidation-Reduction, Rats, Rubidium -- metabolism, Structure-Activity Relationship, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/62275 uri/info:pmid/6985583 uri/info:scp/0018921778 1363723454 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363723454 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |