مورد إلكتروني
A potential role for hydrocortisone in the positive regulation of IL-15-activated NK-cell proliferation and survival.
| العنوان: | A potential role for hydrocortisone in the positive regulation of IL-15-activated NK-cell proliferation and survival. |
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| المصدر: | Blood, 106 (1 |
| بيانات النشر: | 2005-07 |
| تفاصيل مُضافة: | Perez, Sonia Mahaira, Louisa G Demirtzoglou, Fillio J Sotiropoulou, Panagiota Ioannidis, Panayotis Iliopoulou, Eleni G Gritzapis, Angelos D Sotiriadou, Nectaria N Baxevanis, Constantin N Papamichail, Michael |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Although glucocorticoids (GCs) have been described as acting mainly as anti-inflammatory and immunosuppressive drugs, they may also positively influence the immune system. In the present study, we demonstrate for the first time that hydrocortisone (HC), in synergy with interleukin-15 (IL-15), induces a dramatic increase in the expansion of peripheral blood-derived CD56+ cells, favoring the preferential outgrowth of classical natural killer (CD56+CD3- NK) over CD56+CD3+ natural killer T (NKT) cells. HC plus IL-15-driven CD56+ cells exhibited an increased potential for cytokine production with no impairment in their NK- and lymphokine-activated killer (LAK) activities. Elevated levels of GC-induced leucine zipper protein (GILZ) messenger RNA (mRNA) were detected in both NK and NKT cells cultured with HC and IL-15, in comparison to IL-15 alone. Phosphorylation status of signal transducer and activator of transcription 5 (STAT5) was not affected by the presence of HC in either of the populations. On the contrary, HC differentially affected the IL-2/IL-15R beta- and gamma-chain surface expression and the phosphorylation levels of extracellular signal-regulated kinases 1/2 (ERK1/2) in IL-15-activated NK and NKT cells. Our data ascribe a novel role to GCs on mature NK-cell expansion and function and open new perspectives for their use in cellular adoptive cancer immunotherapy. Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Adoptive Transfer -- methods, Anti-Inflammatory Agents -- pharmacology, Antigens, CD3 -- metabolism, Antigens, CD56 -- metabolism, Burkitt Lymphoma, Cell Division -- drug effects, Cell Survival -- drug effects, Cells, Cultured, DNA-Binding Proteins -- metabolism, Humans, Hydrocortisone -- pharmacology, Interleukin-15 -- metabolism, Interleukin-2 -- metabolism, K562 Cells, Killer Cells, Natural -- cytology -- drug effects -- metabolism, Milk Proteins -- metabolism, Mitogen-Activated Protein Kinase 1 -- metabolism, Mitogen-Activated Protein Kinase 3 -- metabolism, Phosphorylation, RNA, Messenger -- analysis, Receptors, Interleukin-15, Receptors, Interleukin-2 -- genetics, STAT5 Transcription Factor, Trans-Activators -- metabolism, Transcription Factors -- genetics, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/142126 uri/info:doi/10.1182/blood-2004-08-3232 uri/info:pii/2004-08-3232 uri/info:pmid/15755904 1363740174 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363740174 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |