مورد إلكتروني
Pancreatic islet B-cell individual variability rather than subpopulation heterogeneity
| العنوان: | Pancreatic islet B-cell individual variability rather than subpopulation heterogeneity |
|---|---|
| المصدر: | Molecular and cellular endocrinology, 118 (1-2 |
| بيانات النشر: | 1996-04 |
| تفاصيل مُضافة: | Mercan, Dany Malaisse, Willy |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Isolated rat pancreatic islet B-cells loaded with the Ca2+-sensitive fluorochrome Flue-3 were examined by single-step flow cytometry at increasing concentrations of D-glucose (1.0 to 20.0 mM). The near forward scatter of light was unaffected by changes in hexose concentration. The Fluo-3 fluorescent signal slightly decreased when the glucose concentration was raised from 1.0 to 5.0 mM, and progressively increased at higher hexose concentrations. The fluorescence attributable to endogenous NAD(P)H increased dramatically throughout the full range of D-glucose concentration, with a typical sigmoidal concentration-response relationship. No evidence for a bimodal frequency distribution of these variables was found, whether at low or high D-glucose concentrations. The dispersion of individual NAD(P)H measurements, as judged by either their coefficient of variation or the height of their modal peak, was less pronounced at high than at low D-glucose concentrations. These findings document vastly different concentration-response relationships for metabolic and ionic variables in glucose-stimulated B-cells. They confirm that all B-cells do not display an identical behavior, but argue against the existence of subpopulation heterogeneity in their responsiveness to D-glucose. SCOPUS: ar.j info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Flow cytometry, Fluo-3, NAD(P)H autofluorescence, Pancreatic islet B-cells, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 1 full-text file(s): application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/78778 uri/info:doi/10.1016/0303-7207(96)03779-3 uri/info:scp/0029939234 1363750753 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363750753 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |