مورد إلكتروني
Human mammary epithelial cells exhibit a differential p53-mediated response following exposure to ionizing radiation or UV light.
| العنوان: | Human mammary epithelial cells exhibit a differential p53-mediated response following exposure to ionizing radiation or UV light. |
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| المؤلفون: | Meyer, KM |
| المصدر: | Oncogene; vol 18, iss 42, 5795-5805; 0950-9232 |
| بيانات النشر: | eScholarship, University of California 1999-10-01 |
| تفاصيل مُضافة: | Meyer, KM Hess, SM Tlsty, TD Leadon, SA |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The tumor suppressor protein, p53, plays a critical role as a transcriptional activator of downstream target genes involved in the cellular response to DNA damaging agents. We examined the cell cycle checkpoint response of human mammary epithelial cells (HMEC) and their isogenic fibroblast counterparts to ionizing (IR) and ultraviolet (UV) radiation, two genotoxic agents whose DNA damage response pathways involve p53. Using flow cytometric analysis, we found that both mortal and immortalized HMEC, which contain wild-type p53 sequence, do not exhibit a G1 arrest in response to IR, but show an intact G2 checkpoint. Supportive evidence from Western analyses revealed that there was neither an increase in p53 nor one of its downstream targets, p21WAF1, in HMEC exposed to IR. In contrast, isogenic mammary fibroblasts arrest at the G1 checkpoint and induce the p53 and p21WAF1 proteins following IR. By comparison, HMEC exposed to UV displayed an S phase arrest and induced the expression of p53 and p21WAF1. Our results show that the cellular response to DNA damage depends on both the type of damage introduced into the DNA and the specific cell type. |
| مصطلحات الفهرس: | Breast, Cells, Cultured, Cell Line, Transformed, Fibroblasts, Epithelial Cells, Humans, Cyclins, Ultraviolet Rays, Cell Cycle, G1 Phase, Gamma Rays, Radiation Tolerance, Female, Tumor Suppressor Protein p53, Cyclin-Dependent Kinase Inhibitor p21, Non-programmatic, p53, p21(WAF1), ionizing radiation, ultraviolet radiation, breast, Breast Cancer, Genetics, Cancer, 2.1 Biological and endogenous factors, Aetiology, 1.1 Normal biological development and functioning, Underpinning research, Clinical Sciences, Oncology and Carcinogenesis, Oncology & Carcinogenesis, article |
| URL: | |
| الإتاحة: | Open access content. Open access content public |
| ملاحظة: | application/pdf Oncogene vol 18, iss 42, 5795-5805 0950-9232 |
| أرقام أخرى: | CDLER oai:escholarship.org:ark:/13030/qt39v5247d qt39v5247d https://escholarship.org/uc/item/39v5247d https://escholarship.org/ 1367476248 |
| المصدر المساهم: | UC MASS DIGITIZATION From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1367476248 |
| قاعدة البيانات: | OAIster |
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