مورد إلكتروني
Transcriptome analysis reveals modulation of human stem cells from the Apical Papilla by species associated with dental root canal infection
| العنوان: | Transcriptome analysis reveals modulation of human stem cells from the Apical Papilla by species associated with dental root canal infection |
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| بيانات النشر: | Umeå universitet, Institutionen för odontologi Umeå universitet, Anatomi Umeå universitet, Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM) Department of Microbiology, Virology and Biotechnology, Mechnikov National University, Odesa, Ukraine Department of Endodontics, Region of Västerbotten, Umeå, Sweden National Bioinformatics Infrastructure Sweden (NBIS), Lund University, Lund, Sweden 2022 |
| تفاصيل مُضافة: | Razghonova, Yelyzaveta Zymovets, Valeriia Wadelius, Philip Rakhimova, Olena Manoharan, Lokeshwaran Brundin, Malin Kelk, Peyman Romani Vestman, Nelly |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Interaction of oral bacteria with stem cells from the apical papilla (SCAP) can negatively affect the success of regenerative endodontic treatment (RET). Through RNA-seq transcriptomic analysis, we studied the effect of the oral bacteria Fusobacterium nucleatum and Enterococcus faecalis, as well as their supernatants enriched by bacterial metabolites, on the osteo- and dentinogenic potential of SCAPs in vitro. We performed bulk RNA-seq, on the basis of which differential expression analysis (DEG) and gene ontology enrichment analysis (GO) were performed. DEG analysis showed that E. faecalis supernatant had the greatest effect on SCAPs, whereas F. nucleatum supernatant had the least effect (Tanimoto coefficient = 0.05). GO term enrichment analysis indicated that F. nucleatum upregulates the immune and inflammatory response of SCAPs, and E. faecalis suppresses cell proliferation and cell division processes. SCAP transcriptome profiles showed that under the influence of E. faecalis the upregulation of VEGFA, Runx2, and TBX3 genes occurred, which may negatively affect the SCAP’s osteo- and odontogenic differentiation. F. nucleatum downregulates the expression of WDR5 and TBX2 and upregulates the expression of TBX3 and NFIL3 in SCAPs, the upregulation of which may be detrimental for SCAPs’ differentiation potential. In conclusion, the present study shows that in vitro, F. nucleatum, E. faecalis, and their metabolites are capable of up- or downregulating the expression of genes that are necessary for dentinogenic and osteogenic processes to varying degrees, which eventually may result in unsuccessful RET outcomes. Transposition to the clinical context merits some reservations, which should be approached with caution. |
| مصطلحات الفهرس: | dentinogenesis, differential gene expression analysis (DEG), Enterococcus faecalis, Fusobacterium nucleatum, osteogenesis, regenerative endodontic treatment (RET), stem cells from the apical papilla (SCAP), transcriptome analysis, Dentistry, Odontologi, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.3390.ijms232214420 |
| URL: | International Journal of Molecular Sciences, 1661-6596, 2022, 23:22 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | UPE oai:DiVA.org:umu-201582 0000-0002-3536-4467 0000-0002-8747-3307 0000-0003-1594-1738 0000-0002-5674-8179 doi:10.3390/ijms232214420 PMID 36430898 ISI:000887457400001 Scopus 2-s2.0-85142777211 1372226021 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1372226021 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.3390.ijms232214420 |
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