مورد إلكتروني
Host-parasite interactions in space and time
| العنوان: | Host-parasite interactions in space and time |
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| بيانات النشر: | Stockholms universitet, Institutionen för molekylär biovetenskap, Wenner-Grens institut Stockholm : Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University 2023 |
| تفاصيل مُضافة: | Hildebrandt, Franziska |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Unicellular parasites of the apicomplexan phylum have a considerable effect on global health and agriculture. Two prominent examples of this phylum include malaria causing parasites of the Plasmodium genus and the widely prevalent parasite Toxoplasma gondii. While sharing a common ancestor, these parasites occupy unique biological niches, follow distinct life cycles, and result in different courses and outcomes of disease. In response to the parasite, the mammalian host has developed efficient and effective defense strategies. However, both Plasmodium and Toxoplasma have evolved strategies to evade the host’s defense response. Plasmodium parasites infect distinct tissues and cell types whereas T. gondii parasites are highly promiscuous and infect all nucleated cells. The identification of key factors involved in the interaction between the host and parasite is crucial for disease intervention, prevention, and eventually eradication efforts. Next-generation sequencing technologies have proven effective tools to investigate the response in a tissue or cell population of an infected organism. Novel genomics methods such as single-cell RNA-seq and spatial transcriptomics have enabled the investigation of heterogeneous transcriptional responses of individual cells in a population as well as heterogeneous expression profiles at spatially distinct tissue positions across entire tissue sections. This thesis pioneers the exploration of these methods in discerning the enormous complexity underlying host-parasite interplay. In Paper I, we determine spatial components of naive mouse liver in its true tissue context. We define gene expression gradients of pericentral and periportal zones in the liver and predict vein types with ambiguous annotations, based on in situ transcriptional profiles. We further identify novel spatial structures with distinct transcriptional profiles, associated with tissue integrity and integrate cell type proportions across the tissue. In Paper II we i |
| مصطلحات الفهرس: | Malaria, liver-stage infection, Toxoplasma, host-parasite interactions, spatial transcriptomics, single-cell omics, single-cell Dual-seq, Genetics, Genetik, Immunology, Immunologi, Microbiology, Mikrobiologi, Bioinformatics (Computational Biology), Bioinformatik (beräkningsbiologi), Biochemistry and Molecular Biology, Biokemi och molekylärbiologi, Doctoral thesis, comprehensive summary, info:eu-repo/semantics/doctoralThesis, text |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | UPE oai:DiVA.org:su-219780 0000-0002-2673-1704 urn:isbn:978-91-8014-426-1 urn:isbn:978-91-8014-427-8 1399998909 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1399998909 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |