مورد إلكتروني
c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
| العنوان: | c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication. |
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| بيانات النشر: | Oxford University Press 1987-06-25 |
| تفاصيل مُضافة: | Iguchi-Ariga, Sanae M. M. Itani, Teru Yamaguchi, Masamitsu Ariga, Hiroyoshi |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication. |
| مصطلحات الفهرس: | Animals, Antibodies, Monoclonal/immunology, Antigens, Polyomavirus Transforming, Antigens, Viral, Tumor/physiology, Cell Line, Cercopithecus aethiops, DNA Replication/drug effects, DNA, Recombinant/biosynthesis, DNA, Viral/biosynthesis, DNA, Viral/genetics, DNA-Binding Proteins/pharmacology, Humans, Oncogene Proteins, Viral/physiology, Plasmids, Proto-Oncogene Proteins/immunology, Proto-Oncogene Proteins/pharmacology, Proto-Oncogene Proteins c-myc, Recombinant Proteins/pharmacology, Simian virus 40/genetics, Transfection, 499, article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | English |
| أرقام أخرى: | YX@ oai:eprints.lib.hokudai.ac.jp:2115/54049 Nucleic acids research, 15(12): 4889-4899 1400957749 |
| المصدر المساهم: | HOKKAIDO UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1400957749 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |