مورد إلكتروني
Production of a robust nanobiocatalyst for municipal wastewater treatment
| العنوان: | Production of a robust nanobiocatalyst for municipal wastewater treatment |
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| المؤلفون: | Hommes, G., Gasser, C.A., Howald, C.B.C., Goers, R., Schlosser, Dietmar, Shahgaldian, P., Corvini, P.F.-X. |
| المصدر: | ISSN: 0960-8524 |
| بيانات النشر: | Elsevier 2012 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Immobilization is a fundamental method to improve both enzyme activity and stability. In the present work, the process previously described for immobilizing laccase – an enzyme oxidizing phenolic compounds – onto fumed silica was optimized, in order to efficiently produce industrially relevant amounts of a nanobiocatalyst for biological micropollutant elimination, whilst saving 80% of surface modification agent (3-aminopropyl triethoxy silane) and 90% of cross-linker (glutaraldehyde). Minimized losses during preparation and favorable effects of immobilization yielded conjugates with drastically increased enzymatic activity (164% of invested activity). Long-term stability and activity regarding bisphenol A (2,2-bis(4-hydroxyphenyl)propane) removal of the synthesized biocatalyst were assessed under application-relevant conditions. With 81.1 ± 0.4% residual activity after 7 days, stability of conjugates was drastically higher than of free laccase, which showed virtually no activity after 1.5 days. These results illustrate the huge potential of fumed silica nanoparticles/laccase-composites for innovative biological wastewater treatment. |
| مصطلحات الفهرس: | laccase, fumed silica nanoparticles, immobilization, phenolic micropollutant, wastewater treatment |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | ISSN: 0960-8524 Bioresource Technology 115 (July 2012);; 8 - 15 English |
| أرقام أخرى: | DEPKD oai:ufz.de:12585 1406003266 |
| المصدر المساهم: | PUBLIKATIONS-DATENBANK From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1406003266 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |