مورد إلكتروني
Revisiting the truncated lamin A produced by a commonly used strain of Lmna knockout mice.
| العنوان: | Revisiting the truncated lamin A produced by a commonly used strain of Lmna knockout mice. |
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| المؤلفون: | Kim, Joonyoung |
| المصدر: | Nucleus; vol 14, iss 1 |
| بيانات النشر: | eScholarship, University of California 2023-12-01 |
| تفاصيل مُضافة: | Kim, Joonyoung Kim, Paul Presnell, Ashley Tu, Yiping Young, Stephen |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The Lmna knockout mouse (Lmna-/-) created by Sullivan and coworkers in 1999 has been widely used to examine lamin A/C function. The knockout allele contains a deletion of Lmna intron 7-exon 11 sequences and was reported to be a null allele. Later, Jahn and coworkers discovered that the mutant allele produces a 54-kDa truncated lamin A and identified, by RT-PCR, a Lmna cDNA containing exon 1-7 + exon 12 sequences. Because exon 12 encodes prelamin As CaaX motif, the mutant lamin A is assumed to be farnesylated. In the current study, we found that the truncated lamin A in Lmna-/- mouse embryonic fibroblasts (MEFs) was predominantly nucleoplasmic rather than at the nuclear rim, leading us to hypothesize that it was not farnesylated. Our study revealed that the most abundant Lmna transcripts in Lmna-/- MEFs contain exon 1-7 but not exon 12 sequences. Exon 1-7 + exon 12 transcripts were detectable by PCR but in trace amounts. We suspect that these findings explain the nucleoplasmic distribution of the truncated lamin A in Lmna-/- MEFs, and subsequent cell transduction experiments support this suspicion. A truncated lamin A containing exon 1-7 sequence was nucleoplasmic, whereas a lamin A containing exon 1-7 + exon 12 sequences was located along the nuclear rim. Our study explains the nucleoplasmic targeting of truncated lamin A in Lmna-/- MEFs and adds to our understanding of a commonly used strain of Lmna-/- mice. |
| مصطلحات الفهرس: | LMNA deficiency, Lamins, lmna transcripts, nuclear envelope, nuclear lamina, protein farnesylation, Animals, Mice, Cell Nucleus, Fibroblasts, Lamin Type A, Knockout, article |
| URL: | |
| الإتاحة: | Open access content. Open access content public |
| ملاحظة: | application/pdf Nucleus vol 14, iss 1 |
| أرقام أخرى: | CDLER oai:escholarship.org:ark:/13030/qt3m08291n qt3m08291n https://escholarship.org/uc/item/3m08291n https://escholarship.org/ 1410331131 |
| المصدر المساهم: | UC MASS DIGITIZATION From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1410331131 |
| قاعدة البيانات: | OAIster |
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