مورد إلكتروني
In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives
| العنوان: | In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives |
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| المصدر: | Chemical and Biological Technologies in Agriculture |
| بيانات النشر: | Springer Science and Business Media Deutschland GmbH 2023-12 |
| تفاصيل مُضافة: | Petrov Ivanković, Anja Milivojević, Ana Ćorović, Marija Simović, Milica Banjanac, Katarina Jansen, Patrick Vukoičić, Ana van den Bogaard, Ellen Bezbradica, Dejan |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant |
| مصطلحات الفهرس: | Blackcurrant, Polyphenols, Enzyme-assisted extraction, Cutaneous microbiota, Skin prebiotic, article |
| URL: | info:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS info:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS info:eu-repo/grantAgreement/ScienceFundRS/Ideje/7750109/RS Horizon Europe 2021–2027 research and innovation programme under grant agreement ID 101060130 (TwinPrebioEnz) |
| الإتاحة: | Open access content. Open access content https://creativecommons.org/licenses/by/4.0 BY openAccess |
| ملاحظة: | Chemical and Biological Technologies in Agriculture English |
| أرقام أخرى: | RSPST oai:TechnoRep.tmf.bg.ac.rs:123456789/6870 2196-5641 10.1186/s40538-023-00502-8 2-s2.0-85176272871 1414439247 |
| المصدر المساهم: | UIVERSITY OF BELGRADE From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1414439247 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |