مورد إلكتروني
Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells
| العنوان: | Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells |
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| بيانات النشر: | Nature Publishing Group country:GB 2011 |
| تفاصيل مُضافة: | Lister, R Pelizzola, M Kida, Y Hawkins, R Nery, J Hon, G Antosiewicz-Bourget, J O'Malley, R Castanon, R Klugman, S Downes, M Yu, R Stewart, R Ren, B Thomson, J Evans, R Ecker, J Lister R Pelizzola M Kida YS Hawkins RD Nery JR Hon G Antosiewicz-Bourget J O'Malley R Castanon R Klugman S Downes M Yu R Stewart R Ren B Thomson JA Evans RM Ecker JR |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Induced pluripotent stem cells (iPSCs) offer immense potential for regenerative medicine and studies of disease and development. Somatic cell reprogramming involves epigenomic reconfiguration, conferring iPSCs with characteristics similar to embryonic stem (ES) cells. However, it remains unknown how complete the reestablishment of ES-cell-like DNA methylation patterns is throughout the genome. Here we report the first whole-genome profiles of DNA methylation at single-base resolution in five human iPSC lines, along with methylomes of ES cells, somatic cells, and differentiated iPSCs and ES cells. iPSCs show significant reprogramming variability, including somatic memory and aberrant reprogramming of DNA methylation. iPSCs share megabase-scale differentially methylated regions proximal to centromeres and telomeres that display incomplete reprogramming of non-CG methylation, and differences in CG methylation and histone modifications. Lastly, differentiation of iPSCs into trophoblast cells revealed that errors in reprogramming CG methylation are transmitted at a high frequency, providing an iPSC reprogramming signature that is maintained after differentiation. |
| مصطلحات الفهرس: | DNA methylation, DNA methylome, stem cells, iPSC, pluripotency, differentiation, non-CpG methylation, high-throughput sequencing, bisulfite sequencing, BIO/11 - BIOLOGIA MOLECOLARE, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/pmid/21289626 info:eu-repo/semantics/altIdentifier/wos/WOS:000287924100035 volume:471 issue:7336 firstpage:68 lastpage:73 numberofpages:6 journal:NATURE |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/closedAccess |
| ملاحظة: | STAMPA English |
| أرقام أخرى: | ITBAO oai:boa.unimib.it:10281/447378 10.1038/nature09798 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-79952264847 1415731738 |
| المصدر المساهم: | BICOCCA OPEN ARCH From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1415731738 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |