مورد إلكتروني
Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe
| العنوان: | Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe |
|---|---|
| المؤلفون: | Monopoli, Marco P., Zendrini, Andrea, Wu, Dan, Cheung, Shane, Sampedro, Gonzalo, Ffrench, Brendan, Nolan, John, Piskareva, Olga, Stalings, Raymond L., Ducoli, Serena, Bergese, Paolo, O’Shea, Donal F. |
| بيانات النشر: | Royal Society of Chemistry 2024-01-25T15:12:01Z 2024-01-25T15:12:01Z 2018-12-28 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The recognition of the biological, diagnostic andmedical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular vesicles in ways that do not alter their inherent characteristics. We report for the first time an endogenous method to NIR-fluorescent labelled exosomes using an amphiphilic probe without the need for immunolabelling or synthetic or chromatographic manipulation of exosomes. Comparative analyses of labelled and unlabelled exosomes with NTA, AFM, flow cytometry and immunoblot analysis all show a high degree of similarity. Spectroscopic analysis and fluorescence imaging confirmed the ability to visualise purified NIR-exosomes. Science Foundation Ireland Depto. de Química Orgánica Fac. de Ciencias Químicas TRUE pub |
| مصطلحات الفهرس: | 547, Aza Compounds, Boron Compounds, Cell Line, Tumor, Exosomes, Fluorescent Dyes, Humans, Infrared Rays, Optical Imaging, Porphobilinogen, Surface-Active Agents, Química orgánica (Química), 23 Química, journal article, AM |
| URL: | 11/PI/1071(T) |
| الإتاحة: | Open access content. Open access content open access |
| ملاحظة: | application/pdf 1364-548X English |
| أرقام أخرى: | ESRCM oai:docta.ucm.es:20.500.14352/95510 1359-7345 1364-548X 10.1039/c8cc02135j 1429625807 |
| المصدر المساهم: | REPOSITORIO E-PRINTS UNIVERSIDAD COMPLU From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1429625807 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |