Varietal effects of barley carbohydrate composition on digestibility, fermentability and microbial ecophysiology in an in vitro model of the pig gastrointestinal tract.
التفاصيل البيبلوغرافية
العنوان:
Varietal effects of barley carbohydrate composition on digestibility, fermentability and microbial ecophysiology in an in vitro model of the pig gastrointestinal tract.
Varietal effects of barley carbohydrate composition on digestibility, fermentability and microbial ecophysiology in an in vitro model of the pig gastrointestinal tract. Journal of Animal Science, 87(E-Suppl. 3), 93.Savoy, IL, USAAmerican Society of Animal Science. (2009).
Carbohydrate (CHO) composition can vary markedly between barleyvarieties. Their influence on digestibility, intestinal fermentation andmicrobiota in pigs was studied in vitro. Ten hulless (HLB) and 6hulled barleys (HB) differing in B-glucan, non-starch polysaccharides(NSP), starch content, and amylose/amylopectin ratio, were hydrolyzedenzymatically and subsequently fermented for 72h. CHO fermentationkinetics were modeled; microbial composition and short-chain fattyacid (SCFA) production were analyzed.In HLB, in vitro DM digestibility was positively correlated to starchand amylopectin content and CP digestibility to amylopectin (P<0.05),whereas both were negatively correlated to insoluble NSP (P<0.05). Rateof fermentation was different (P<0.01) between barley types but notcorrelated to the CHO composition. However, high B-glucan contentsinduced faster fermentation (P<0.05, HLB; P<0.10, HB). SCFA molarratios after fermentation of HLB were higher in propionate and branchedchainfatty acids and lower in acetate compared to HB (P<0.01). WithHLB, amylose content was positively correlated to butyrate productionand negatively to propionate, which was positively correlated to solubleNSP content (P<0.01). In HB, no correlation between SCFA productionand the carbohydrate composition was found. TRFLP analysisrevealed that Bacteroides and members of Clostridium cluster XIVawere differentially affected in HLB compared to HB as well as bythe type and source of CHO. Microbial profiles were also correlated(P<0.05) to SCFA and fermentation parameters but response differedsignificantly between HB and HLB. The strongest correlation betweenCHO structure, microbial abundance and fermentation parameters wasevident in HLB. Hulless barleys may offer the greatest opportunity toimprove gut health in pigs.
نوع الوثيقة:
conferenceObject
اللغة:
English
Relation:
ASAS/ADSA Midwest Meeting, Des Moines, USA (From 16-3-2009 to 18-3-2009)
