دورية أكاديمية
Polycytidylation of mitochondrial mRNAs in Chlamydomonas reinhardtii
| العنوان: | Polycytidylation of mitochondrial mRNAs in Chlamydomonas reinhardtii |
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| المؤلفون: | Salinas-Giegé, T., Cavaiuolo, M., Cognat, V., Ubrig, E., Remacle, Claire, Duchêne, A.-M., Vallon, O., Maréchal-Drouard, L. |
| المصدر: | Nucleic Acids Research, 45 (22), 12963-12973 (2017) |
| بيانات النشر: | Oxford University Press, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Chlamydomonas reinhardtii, Base Sequence, Chlorophyta, Genome, Mitochondrial, Mitochondria, Phylogeny, Poly C, RNA, Messenger, Sequence Homology, Nucleic Acid, Transcription, Genetic, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | The unicellular photosynthetic organism, Chlamydomonas reinhardtii, represents a powerful model to study mitochondrial gene expression. Here, we show that the 5'- and 3'-extremities of the eight Chlamydomonas mitochondrial mRNAs present two unusual characteristics. First, all mRNAs start primarily at the AUG initiation codon of the coding sequence which is often marked by a cluster of small RNAs. Second, unusual tails are added post-transcriptionally at the 3'-extremity of all mRNAs. The nucleotide composition of the tails is distinct from that described in any other systems and can be partitioned between A/U-rich tails, predominantly composed of Adenosine and Uridine, and C-rich tails composed mostly of Cytidine. Based on 3' RACE experiments, 22% of mRNAs present C-rich tails, some of them composed of up to 20 consecutive Cs. Polycytidylation is specific to mitochondria and occurs primarily on mRNAs. This unprecedented post-transcriptional modification seems to be a specific feature of the Chlorophyceae class of green algae and points out the existence of novel strategies in mitochondrial gene expression. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article |
| اللغة: | English |
| Relation: | urn:issn:0305-1048; urn:issn:1362-4962 |
| DOI: | 10.1093/nar/gkx903 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/229179 |
| حقوق: | restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
| رقم الأكسشن: | edsorb.229179 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1093/nar/gkx903 |
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