دورية أكاديمية
Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrheic and septicemic calves and relatedness to human Shigatoxigenic E. coli O80:H2
| العنوان: | Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrheic and septicemic calves and relatedness to human Shigatoxigenic E. coli O80:H2 |
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| المؤلفون: | Habets, Audrey, Crombé, F., Nakamura, K., Guérin, Virginie, De Rauw, K., Piérard, D., Saulmont, M., Hayashi, T., Mainil, Jacques, Thiry, Damien |
| المصدر: | Journal of Applied Microbiology, 130 (1), 258-264 (2021-01) |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | enteropathogenic Escherichia coli, calves, genome sequencing, phylogenetic analysis, serotype O80:H2, Shigatoxigenic Escherichia coli, Life sciences, Veterinary medicine & animal health, Sciences du vivant, Médecine vétérinaire & santé animale |
| الوصف: | Aim: The purpose of this work was to identify and genetically characterizeenterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli(EPEC) O80:H2 from diarrhoeic and septicaemic calves in Belgium and tocomparing them with human EHEC after whole genome sequencing.Methods and Results: Ten EHEC and 21 EPEC O80 identified by PCRbetween 2009 and 2018 from faeces, intestinal content and a kidney ofdiarrhoeic or septicaemic calves were genome sequenced and compared to 19human EHEC identified between 2008 and 2019. They all belonged to the O80:H2 serotype and ST301, harboured the eaeξ gene, and 23 of the 29 EHECcontained the stx2d gene. Phylogenetically, they were distributed in two majorsub-lineages: one comprised a majority of bovine EPEC whereas the secondone comprised a majority of stx2d bovine and human EHEC.Conclusions: Not only EPEC but also EHEC O80:H2 are present in diarrhoeicand septicaemic calves in Belgium and are genetically related to human EHEC.Significance and Impact of the Study: These findings support the need toassess cattle as potential source of contamination of humans by EHEC O80:H2and to understand the evolution of bovine and human EHEC and EPEC O80:H2. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article |
| اللغة: | English |
| Relation: | urn:issn:1364-5072; urn:issn:1365-2672 |
| DOI: | 10.1111/JAM.14759 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/250752 |
| حقوق: | restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
| رقم الأكسشن: | edsorb.250752 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1111/JAM.14759 |
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