دورية أكاديمية
Escherichia coli O80 in Healthy Cattle: Absence of Shigatoxigenic and Enteropathogenic E. coli O80:H2 and (Phylo) Genomics of Non-Clonal Complex 165 E. coli O80
| العنوان: | Escherichia coli O80 in Healthy Cattle: Absence of Shigatoxigenic and Enteropathogenic E. coli O80:H2 and (Phylo) Genomics of Non-Clonal Complex 165 E. coli O80 |
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| المؤلفون: | Ikeda, Rie, Nakamura, Keiji, Saulmont, Ma, Habe, Audrey, Duprez, Jean-Noël, Korsak, Nicolas, Hayashi, Tetsuya, Thiry, Damien, Mainil, Jacques |
| المصدر: | Microorganisms, 11 (2), 230 (2023-01-17) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | healthy cattle, Shigatoxigenic Escherichia coli, enteropathogenic Escherichia coli, O80:H2, O80:H6, O80:H45, CC165, phylogenomics, Life sciences, Veterinary medicine & animal health, Sciences du vivant, Médecine vétérinaire & santé animale |
| الوصف: | The origin of human and calf infections by Shigatoxigenic (STEC) and enteropathogenic(EPEC) Escherichia coli O80:H2 is still unknown. The aim of this study was to identify E. coli O80 inhealthy cattle with an emphasis on melibiose non-fermenting E. coli O80:H2. Faecal materials collectedfrom 149 bulls at 1 slaughterhouse and 194 cows on 9 farms were tested with O80 antigen-encodinggene PCR after overnight growth in enrichment broths. The 53 O80 PCR-positive broths were streakedon different (semi-)selective agar plates. Five E. coli colonies from 3 bulls and 11 from 2 cows testedpositive with the O80 PCR, but no melibiose non-fermenting E. coli was isolated. However, these16 E. coli O80 were negative with PCR targeting the fliCH2, eae, stx1, stx2 and hlyF genes and wereidentified by WGS to serotypes and sequence types O80:H6/ST8619 and O80:H45/ST4175. Theywere phylogenetically related to E. coli O80:H6 and O80:H45 isolated from different animal species indifferent countries, respectively, but neither to STEC and EPEC O80:H2/ST301, nor to other serotypesof the clonal complex 165. As a conclusion, healthy adult cattle were not identified as a source ofcontamination of humans and calves by STEC or EPEC O80:H2. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| اللغة: | English |
| Relation: | https://www.mdpi.com/2076-2607/11/2/230/pdf; urn:issn:2076-2607 |
| DOI: | 10.3390/microorganisms11020230 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/298997 |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الأكسشن: | edsorb.298997 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.3390/microorganisms11020230 |
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