دورية أكاديمية
Comprehensive Insight into Colorectal Cancer Metabolites and Lipids for Human Serum: A Proof-of-Concept Study.
| العنوان: | Comprehensive Insight into Colorectal Cancer Metabolites and Lipids for Human Serum: A Proof-of-Concept Study. |
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| المؤلفون: | Bhatt, Kinjal, Orlando, Titziana, Meuwis, Marie-Alice, Louis, Edouard, Stefanuto, Pierre-Hugues, Focant, Jean-François |
| المصدر: | International Journal of Molecular Sciences, 24 (11), 9614 (2023-06-01) |
| بيانات النشر: | MDPI, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | GC×GC–TOFMS, blood, chemometrics, colorectal cancer, comprehensive gas chromatography, lipidomics, mass spectrometry, metabolomics, sample preparation, separation science, serum, Fatty Acids, Humans, Gas Chromatography-Mass Spectrometry/methods, Mass Spectrometry/methods, Metabolomics/methods, Colorectal Neoplasms/diagnosis, Catalysis, Molecular Biology, Spectroscopy, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry, Inorganic Chemistry, General Medicine, Physical, chemical, mathematical & earth Sciences, Chemistry, Physique, chimie, mathématiques & sciences de la terre, Chimie |
| الوصف: | Colorectal cancer (CRC) ranks as the third most frequently diagnosed cancer and the second leading cause of cancer-related deaths. The current endoscopic-based or stool-based diagnostic techniques are either highly invasive or lack sufficient sensitivity. Thus, there is a need for less invasive and more sensitive screening approaches. We, therefore, conducted a study on 64 human serum samples representing three different groups (adenocarcinoma, adenoma, and control) using cutting-edge GC×GC-LR/HR-TOFMS (comprehensive two-dimensional gas chromatography coupled with low/high-resolution time-of-flight mass spectrometry). We analyzed samples with two different specifically tailored sample preparation approaches for lipidomics (fatty acids) (25 μL serum) and metabolomics (50 μL serum). In-depth chemometric screening with supervised and unsupervised approaches and metabolic pathway analysis were applied to both datasets. A lipidomics study revealed that specific PUFA (ω-3) molecules are inversely associated with increased odds of CRC, while some PUFA (ω-6) analytes show a positive correlation. The metabolomics approach revealed downregulation of amino acids (alanine, glutamate, methionine, threonine, tyrosine, and valine) and myo-inositol in CRC, while 3-hydroxybutyrate levels were increased. This unique study provides comprehensive insight into molecular-level changes associated with CRC and allows for a comparison of the efficiency of two different analytical approaches for CRC screening using same serum samples and single instrumentation. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| اللغة: | English |
| Relation: | https://www.mdpi.com/1422-0067/24/11/9614/pdf; urn:issn:1661-6596; urn:issn:1422-0067 |
| DOI: | 10.3390/ijms24119614 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/304852 |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الأكسشن: | edsorb.304852 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.3390/ijms24119614 |
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