دورية أكاديمية
Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)
| العنوان: | Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1) |
|---|---|
| المؤلفون: | Chohra, Ilyas, Giri, Subhajit, Malgrange, Brigitte |
| المساهمون: | GIGA Stem Cells-Developmental Neurobiology - ULiège |
| المصدر: | International Journal of Molecular Sciences, 24 (13), 10543 (2023-06-23) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Molecular Biology, crispr, human embryonic stem cells, Human health sciences, Otolaryngology, Life sciences, Biochemistry, biophysics & molecular biology, Sciences de la santé humaine, Oto-rhino-laryngologie, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | The chromatin remodeler Chromodomain-helicase-DNA-binding protein 4 (CHD4) is crucial for the development of multiple organ systems. Functional mutations of CHD4 have recently been described in a developmental disorder, namely Siffrim-Hitz-Weiss syndrome (SIHIWES). Herein, we have generated a homozygous CHD4G1003D hESC line (WAe025-A-1) using CRISPR/eCas9-based gene editing in the WA-25 hESC line. The edited hESC line maintains normal karyotype, pluripotency, and ability to differentiate into three germ layers. This cell line will be a valuable resource for studying the functional role of CHD4 during the development and disease modeling of SIHIWES in vitro. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| اللغة: | English |
| Relation: | https://www.mdpi.com/1422-0067/24/13/10543/pdf; urn:issn:1661-6596; urn:issn:1422-0067 |
| DOI: | 10.3390/ijms241310543 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/305518 |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الأكسشن: | edsorb.305518 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.3390/ijms241310543 |
|---|