مؤتمر
Cortisol and DHEA as markers of placentitis in pregnant mares: a preliminary study
| العنوان: | Cortisol and DHEA as markers of placentitis in pregnant mares: a preliminary study Le Cortisol et la DHEA comme marqueurs de la placentite chez la jument gestante : une étude préliminaire |
|---|---|
| المؤلفون: | Ledeck, Joy, Peeters, Stéphanie, Le Goff, Caroline, Deleuze, Stefan, Cavalier, Etienne, Ponthier, Jérôme |
| المساهمون: | FARAH. Médecine vétérinaire comparée - ULiège, FARAH - Fundamental and Applied Research for Animals and Health - ULiège |
| المصدر: | Journal of Equine Veterinary Science, 125 (2023-06); International Symposium On Equine Reproduction, Foz do Iguaçu, Brazil [BR], 10-14 juillet 2023 |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Cortisol, DHEA, Placentitis, Mares, Liquid chromatography coupled to mass spectrometry, Biomarkers, Life sciences, Veterinary medicine & animal health, Sciences du vivant, Médecine vétérinaire & santé animale |
| الوصف: | Placentitis in mares is a subclinical cause of abortion, high- lighting the importance of early detection for successful treatment. Sexual steroids could serve as biomarkers, but cross-reactivity and a limited detection range of immunoassays may hinder ac- curate measures. This study evaluated some steroids as poten- tial markers of placentitis using liquid chromatography coupled to mass spectrometry (LC-MS), known to improve detection and quantification of steroids with good selectivity, specificity, and allowing multiplexing capabilities (Conley et al., Reproduction. 2019;158:197-208; Ledeck et al., Theriogenology. 2022;189:86- 91). From the 7th month of pregnancy, mares were transrectally scanned to measure the combined thickness of the uterus and placenta (CTUP), and blood samples were collected at a fixed time of day. Ten healthy mares (HM) and nine mares diagnosed with non-experimentally-induced placentitis (PM) between 8 and 10 months were enrolled, based on ultrasonographic placentitis signs: heterogeneous echogenicity and/or thickened CTUP. Post- partum allantochorion examination confirmed the diagnosis. Sub- sequently, PM were further excluded from the study as they re- ceived treatments for placentitis. Serum concentrations of pro- gesterone, 17α-hydroxyprogesterone (17αOHP), dehydroepiandros- terone (DHEA), and cortisol were assayed using LC-MS. Non- normally distributed data were presented as medians, and groups were compared at the same month of pregnancy using the Mann- Whitney test. There were no significant differences in 17αOHP and progesterone concentrations between groups at any month.DHEA concentrations were significantly higher (p=0.0297) at 8 months in PM (3.552μg/L) compared to HM (2.240μg/L). A sim- ilar trend was observed at 9 months, with DHEA concentra- tions of 2.126μg/L and 1.445μg/L in PM and HM, respectively (p=0.0604). In contrast, HM had significantly higher (p=0.022) cortisol concentrations at 7 months (71.75μg/L) compared to PM (38.62μg/L). This difference tended (p=0.0529) to be observed at 8 months with cortisol concentrations of 52.25μg/L (HM) and 37.28μg/L (PM). The DHEA/cortisol ratio was significantly higher in PM than in HM at 9 months (p=0.013) and tended to be in- creased at 7 and 8 months (respectively, p=0.0659 and p=0.0529). Placentitis has been shown to increase pregnenolone production (Ousey et al., Theriogenology. 2005;63:1844-1856). In this prelim- inary study, PM were observed to metabolize pregnenolone into DHEA rather than cortisol, without changes in 17αOHP concen- trations. Reduced cortisol concentrations at 7 months could be an early but non-specific biomarker of placentitis. Conversely, elevated DHEA Ievels only appear together with ultrasonographic signs at 8 months but could become a more specific biomarker, which dif- fers from previous findings in mares with experimentally-induced placentitis (Canisso et al., Equine Veterinary Journal. 2017;49:244- 249). These changes result in a higher DHEA/cortisol ratio in PM at 9 months. However, further research should explore mod- ifications of steroids’ pathways in PM to confirm the value of DHEA and cortisol for early diagnosis of placentitis in mares. Study of equine placental sexual steroids’ metabolism using Mass Spectrometry (LC-MS/MS) and in vitro model to develop early diagnosis methods of placental pathologies |
| نوع الوثيقة: | conference paper http://purl.org/coar/resource_type/c_5794 conferenceObject peer reviewed |
| اللغة: | English |
| Relation: | Cortisol and DHEA as markers of placentitis in pregnant mares: a preliminary study; urn:issn:0737-0806; urn:issn:1542-7412 |
| DOI: | 10.1016/j.jevs.2023.104773 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/305667 |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الأكسشن: | edsorb.305667 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1016/j.jevs.2023.104773 |
|---|