دورية أكاديمية
Diagnosis of psoroptic sheep scab with an improved enzyme-linked immunosorbent assay.
| العنوان: | Diagnosis of psoroptic sheep scab with an improved enzyme-linked immunosorbent assay. |
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| المؤلفون: | Ochs, H., Lonneux, J., Losson, Bertrand, Deplazes, P. |
| المصدر: | Veterinary Parasitology, 96 (3), 233-42 (2001) |
| بيانات النشر: | Elsevier, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Animals, Antibodies/blood/immunology, Antibody Specificity, Antigens/immunology, Enzyme-Linked Immunosorbent Assay/methods/veterinary, Insecticides/therapeutic use, Ivermectin/analogs & derivatives/therapeutic use, Mite Infestations/diagnosis/immunology, Mites/immunology, Rabbits, Sensitivity and Specificity, Sheep, Sheep Diseases/diagnosis/immunology/parasitology, Skin/parasitology, Life sciences, Veterinary medicine & animal health, Sciences du vivant, Médecine vétérinaire & santé animale |
| الوصف: | An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of specific antibodies against crude Psoroptes antigen. The diagnostic sensitivity was 93.7% in 191 sheep with clinical signs associated with mange. These animals originated from 29 flocks in which psoroptic mites were detected. All of 59 sheep infested with Psoroptes ovis were seropositive. Additionally, in 49% of 70 clinically unaffected sheep originating from P. ovis-infested flocks, specific antibodies could be detected, suggesting that asymptomatic infestations can be diagnosed by serology. The specificity of the ELISA was 96.5% as determined with 254 sheep originating from 44 flocks without clinical mange. Cross-reactivity in a low range was detected with selected sera of sheep with clinical chorioptic or forage mite infestations. Four sheep seroconverted 2 weeks after experimental P. ovis infestation, i.e. 2 weeks before clinical signs became obvious. After successful doramectin treatment of 14 sheep with naturally acquired P. ovis infestation, the ELISA values declined slowly but remained positive in seven cases beyond 17 weeks. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article |
| اللغة: | English |
| Relation: | urn:issn:0304-4017; urn:issn:1873-2550 |
| DOI: | 10.1016/S0304-4017(00)00444-1 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/41155 |
| رقم الأكسشن: | edsorb.41155 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1016/S0304-4017(00)00444-1 |
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