دورية أكاديمية
Transferrin-Binding Protein B Of Neisseria Meningitidis: Sequence-Based Identification Of The Transferrin-Binding Site Confirmed By Site-Directed Mutagenesis
| العنوان: | Transferrin-Binding Protein B Of Neisseria Meningitidis: Sequence-Based Identification Of The Transferrin-Binding Site Confirmed By Site-Directed Mutagenesis |
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| المؤلفون: | Renauld-Mongenie, G., Lins, Laurence, Krell, T., Laffly, L., Mignon, M., Dupuy, M., Delrue, Rm., Guinet-Morlot, F., Brasseur, Robert, Lissolo, L. |
| المصدر: | Journal of Bacteriology, 186 (3), 850-857 (2004) |
| بيانات النشر: | American Society for Microbiology, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | A sequence-based prediction method was employed to identify three ligand-binding domains in transferrin-binding protein B (TbpB) of Neisseria meningitidis strain B16B6. Site-directed mutagenesis of residues located in these domains has led to the identification of two domains, amino acids 53 to 57 and 240 to 245, which are involved in binding to human transferrin (htf). These two domains are conserved in an alignment of different TbpB sequences from N. meningitidis and Neisseria gonorrhoeae, indicating a general functional role of the domains. Western blot analysis and BIAcore and isothermal titration calorimetry experiments demonstrated that site-directed mutations in both binding domains led to a decrease or abolition of htf binding. Analysis of mutated proteins by circular dichroism did not provide any evidence for structural alterations due to the amino acid replacements. The TbpB mutant R243N was devoid of any htf-binding activity, and antibodies elicited by the mutant showed strong bactericidal activity against the homologous strain, as well as against several heterologous tbpB isotype I strains. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article |
| اللغة: | English |
| Relation: | urn:issn:0021-9193; urn:issn:1098-5530 |
| DOI: | 10.1128/JB.186.3.850-857.2004 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/63523 |
| حقوق: | restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
| رقم الأكسشن: | edsorb.63523 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1128/JB.186.3.850-857.2004 |
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