دورية أكاديمية
Overexpression of TIMP-1 under the MMP-9 promoter interferes with wound healing in transgenic mice
| العنوان: | Overexpression of TIMP-1 under the MMP-9 promoter interferes with wound healing in transgenic mice |
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| المؤلفون: | Salonurmi, T., Parikka, M., Kontusaari, S., Pirila, E., Munaut, Carine, Salo, T., Tryggvason, K. |
| المصدر: | Cell and Tissue Research, 315 (1), 27-37 (2004-01) |
| بيانات النشر: | Springer-Verlag, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | gene expression regulation, keratinocytes, matrix metalloproteinases, tissue inhibitor of metalloproteinase-1, transgenic mice, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | We have generated transgenic mice harboring the murine matrix metalloproteinase 9 (MMP-9) promoter cloned in front of human TIMP-1 cDNA. The transgenic mice were viable and fertile and exhibited normal growth and general development. During wound healing the mice were shown to express human TIMP-1 in keratinocytes that normally express MMP-9. However, the healing of skin wounds was significantly retarded with slow migration of keratinocytes over the wound in transgenic mice. In situ zymography carried out on wound tissues revealed total blockage of gelatinolytic activity (i.e., MMP-9 and MMP-2). The results confirm studies with MMP-9 knockout mice showing that MMP-9 is not essential for general development, but they also demonstrate an important role of keratinocyte MMP-9, as well that of other keratinocyte MMPs that are inhibited by TIMP-1, in wound healing. The transgenic mice generated in this study provide a model for the role of MMPs in MMP-9-producing cells in other challenging situations such as bone fracture recovery and cancer invasion. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article |
| اللغة: | English |
| Relation: | urn:issn:0302-766X; urn:issn:1432-0878 |
| DOI: | 10.1007/s00441-003-0814-1 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/74669 |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الأكسشن: | edsorb.74669 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1007/s00441-003-0814-1 |
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