دورية أكاديمية
Adhesion of synchronized human hematopoietic progenitor cells to fibronectin and vascular cell adhesion molecule-1 fluctuates reversibly during cell cycle transit in ex vivo culture.
| العنوان: | Adhesion of synchronized human hematopoietic progenitor cells to fibronectin and vascular cell adhesion molecule-1 fluctuates reversibly during cell cycle transit in ex vivo culture. |
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| المؤلفون: | Huygen, Sandra, Giet, Olivier, Artisien, Vincent, Di Stefano, Ivano, Beguin, Yves, Gothot, André |
| المصدر: | Blood, 100 (8), 2744-52 (2002) |
| بيانات النشر: | American Society of Hematology, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Antigens, CD/analysis, Antigens, CD34/analysis, Cell Adhesion/physiology, Cell Culture Techniques/methods, Cell Cycle/physiology, Cells, Cultured, Delivery, Obstetric, Fetal Blood/cytology, Fibronectins/physiology, Hematopoietic Stem Cells/cytology/physiology, Humans, Infant, Newborn, Time Factors, Vascular Cell Adhesion Molecule-1/physiology, Human health sciences, Hematology, Sciences de la santé humaine, Hématologie |
| الوصف: | Ex vivo expansion of hematopoietic stem/progenitor cells may result in defective engraftment. Human cord blood CD34(+) progenitor cells were synchronized and assayed for adhesion and migration onto fibronectin (Fn) and vascular cell adhesion molecule-1 (VCAM-1) at different stages of a first cell cycle executed ex vivo. During S phase transit, adhesion to Fn was transiently increased while binding to VCAM-1 was reversibly decreased, after which adhesion to both ligands returned to baseline levels with cell cycle completion. Transmigration across Fn and VCAM-1 decreased irreversibly during S phase progression. The function of alpha4 and alpha5 integrins was assessed with specific neutralizing antibodies. In uncultured CD34(+) cells and long-term culture-initiating cells (LTC-ICs), both adhesion and migration on Fn were inhibited by anti-alpha4 but not by anti-alpha5 antibodies. In mitotically activated CD34(+) cells and LTC-ICs, adhesion and migration on Fn were mainly dependent on alpha5 integrin and to a lesser extent on alpha4 integrin. Changes in integrin function were not dependent on parallel modulation of integrin expression. In conclusion, Fn and VCAM-1 binding of progenitor cells fluctuates reversibly during cell cycle transit ex vivo. In addition, our data show that mitogenic activation induces a shift from a dominant alpha4 to a preferential alpha5 integrin-dependent interaction with Fn. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article |
| اللغة: | English |
| Relation: | urn:issn:0006-4971; urn:issn:1528-0020 |
| DOI: | 10.1182/blood.V100.8.2744 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/8933 |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الأكسشن: | edsorb.8933 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1182/blood.V100.8.2744 |
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